Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection
Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection
复制标题
蒸发光散射检测高效液相色谱法测定油菜素内酯类似物
DOI：
10.1080/00032719.2014.954123
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发表时间：
2015-03-04
影响因子：
2
通讯作者：
Huang, Qiliang
中科院分区：
文献类型：
--
作者：
Cao, Lidong;Yang, Jing;Huang, Qiliang
建立了一种高效液相色谱蒸发光散射检测器分离测定油菜素类似物24-表油菜素、（22 S，23 S）-24-表油菜素、28-高油菜素和（22 S，23 S）-28-表高油菜素的方法。采用C18反相色谱柱（150 mm × 4.60 mm，3 µm），乙腈-水（38：62，v/v）等度洗脱，柱温30°C。检测器的漂移管温度为60°C，辅助气体（氮气）压力为360 kPa。回归方程在试验范围内呈线性关系（R2 = 0.9984-0.9994）。检测限和定量限分别在0.12 - 0.17 µg和0.24 - 0.33 µg范围内。应用经充分验证的方法定量技术材料和制剂中的活性成分含量，并提供了质量控制的替代方法。
An efficient method based on high-performance liquid chromatography with evaporative light scattering detection was developed for the separation and determination of four brassinolide analogs [24-epibrassinolide, (22S, 23S)-24-epibrassinolide, 28-homobrassinolide, and (22S, 23S)-28-epihomobrassinolide] without prior derivatization. The optimized analysis was carried out on a C18 reversed-phase column (150 mm × 4.60 mm, 3 µm) at 30°C using isocratic elution of acetonitrile and water (38:62, v/v). The drift tube temperature of the detector was 60°C and the auxiliary gas (nitrogen) pressure was 360 kPa. The regression equations revealed linear relationships (R2 = 0.9984–0.9994) within the test ranges. The limits of detection and quantification were in the ranges of 0.12 to 0.17 µg and 0.24 to 0.33 µg, respectively. The fully validated method was applied to quantify the active ingredient content in technical material and formulations and provides an alternative approach for quality control.