A Plasma Membrane Receptor Kinase, GHR1, Mediates Abscisic Acid- and Hydrogen Peroxide-Regulated Stomatal Movement in Arabidopsis
A Plasma Membrane Receptor Kinase, GHR1, Mediates Abscisic Acid- and Hydrogen Peroxide-Regulated Stomatal Movement in Arabidopsis
复制标题
拟南芥质膜受体激酶 GHR1 介导脱落酸和过氧化氢调节的气孔运动
DOI：
10.1105/tpc.112.100107
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发表时间：
2012-06-01
期刊：
影响因子：
11.6
通讯作者：
Gong, Zhizhong
中科院分区：
文献类型：
--
作者：
Hua, Deping;Wang, Cun;Gong, Zhizhong
植物激素脱落酸（阿坝）调节气孔运动在干旱胁迫下，这种调节需要过氧化氢（H2 O2）。我们分离了保卫细胞抗过氧化氢1（GHR 1），它编码一种位于拟南芥质膜上的受体样激酶。ghr 1突变体是阿坝和H2 O2诱导气孔关闭的缺陷型。遗传分析表明，GHR 1是阿坝信号传导的关键早期组分。ghr 1突变损害阿坝和H2 O2调节的S-型阴离子电流的激活在保卫细胞。此外，GHR 1物理相互作用，磷酸化，并激活S-型阴离子通道慢阴离子通道相关的1时，共表达在非洲爪蟾卵母细胞，这种激活被抑制ABA-不敏感2（ABI 2），而不是ABI 1。我们的研究确定了阿坝和H2 O2信号的关键组成部分，参与气孔运动，并解决了一个长期存在的谜团ABI 1和ABI 2在这个过程中的差异功能。
The plant hormone abscisic acid (ABA) regulates stomatal movement under drought stress, and this regulation requires hydrogen peroxide (H2O2). We isolated GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1), which encodes a receptor-like kinase localized on the plasma membrane in Arabidopsis thaliana. ghr1 mutants were defective ABA and H2O2 induction of stomatal closure. Genetic analysis indicates that GHR1 is a critical early component in ABA signaling. The ghr1 mutation impaired ABA- and H2O2-regulated activation of S-type anion currents in guard cells. Furthermore, GHR1 physically interacted with, phosphorylated, and activated the S-type anion channel SLOW ANION CHANNEL-ASSOCIATED1 when coexpressed in Xenopus laevis oocytes, and this activation was inhibited by ABA-INSENSITIVE2 (ABI2) but not ABI1. Our study identifies a critical component in ABA and H2O2 signaling that is involved in stomatal movement and resolves a long-standing mystery about the differential functions of ABI1 and ABI2 in this process.