Fascin as a Useful Marker for Identifying Neural Components in Immature Teratomas of Human Ovary and Those Derived From Murine Embryonic Stem Cells
Fascin as a Useful Marker for Identifying Neural Components in Immature Teratomas of Human Ovary and Those Derived From Murine Embryonic Stem Cells
复制标题
肌成束蛋白作为识别人卵巢未成熟畸胎瘤和鼠胚胎干细胞衍生的神经成分的有用标记物
DOI：
10.1097/pgp.0000000000000528
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kuroda M.
中科院分区：
文献类型：
--
作者：
Umehara R;Kurata A;Takanashi M;Hashimoto H;Fujita K;Nagao T;Kuroda M.
人类卵巢未成熟畸胎瘤是一种罕见的疾病，其诊断和分级目前是基于肿瘤中未成熟神经成分的存在和数量的组织学评估。尽管肿瘤分级很重要，但未成熟的神经成分，特别是没有玫瑰花结形成的神经成分很难识别，部分原因是目前还没有有用的生物标志物。为此，我们研究了16个来自人类卵巢的未成熟畸胎瘤，以及10个来自小鼠胚胎干细胞移植到免疫缺陷小鼠的畸胎瘤。对细胞角蛋白、胶质纤维酸性蛋白、S100和筋膜蛋白进行免疫组化。结果表明，尽管在成熟神经组织中检测到胶质纤维酸性蛋白和S100的表达，但在来自人卵巢和胚胎干细胞的未成熟畸胎瘤的未成熟神经成分中均未观察到它们的表达。相比之下，在来自人卵巢和胚胎干细胞的未成熟畸胎瘤中，无论玫瑰花结形成与否，在成熟和未成熟的神经成分中都明显发现了束蛋白免疫阳性。与苏木精和伊红染色相比，用束蛋白免疫染色评估未成熟神经成分的数量相同或略有增加。这些结果表明，束蛋白免疫染色可作为一种生物标志物，用于正确诊断和分级人类未成熟畸胎瘤。此外，通过准确评估移植到免疫缺陷小鼠体内的多能干细胞源性肿瘤的成熟状态，束蛋白免疫染色可能有助于再生医学的发展。
Immature teratoma of the human ovary is a rare disease, and its diagnosis and grading are currently based on histologic evaluation of the presence and amount of immature neural components in the tumor. Despite the importance of tumor grading, immature neural components especially without rosette formation are difficult to identify, partly because useful biomarkers for them are not yet available. Toward this goal, we investigated 16 immature teratomas from human ovaries as well as 10 of those derived from murine embryonic stem cells transplanted into immunodeficient mice. Immunohistochemistry was performed for cytokeratin, glial fibrillary acidic protein, S100, and fascin. It was demonstrated that glial fibrillary acidic protein and S100 expression was not observed in the immature neural components of immature teratomas derived from both human ovary and embryonic stem cells, although their expression was detected in mature neural tissues. In contrast, fascin immunopositivity was clearly found in both mature and immature neural components regardless of rosette formation in immature teratomas derived from both human ovary and embryonic stem cells. Assessment of immature neural components by fascin immunostaining yielded the same or slightly increased quantity than quantification based on hematoxylin and eosin staining. These results suggest that fascin immunostaining is useful as a biomarker in correctly diagnosing and grading human immature teratomas. Further, fascin immunostaining may contribute to the development of regenerative medicine through accurate assessment of the maturation status of pluripotent stem cell-derived tumors transplanted into immunodeficient mice.