BEHAB, a new member of the proteoglycan tandem repeat family of hyaluronan-binding proteins that is restricted to the brain.
BEHAB, a new member of the proteoglycan tandem repeat family of hyaluronan-binding proteins that is restricted to the brain.
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DOI：
10.1083/jcb.125.2.495
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发表时间：
1994-04
期刊：
影响因子：
--
通讯作者：
Hockfield S
中科院分区：
文献类型：
--
作者：
Jaworski DM;Kelly GM;Hockfield S
透明质酸（HA）是所有组织细胞外基质中普遍存在的成分。在哺乳动物中枢神经系统（CNS）中，血凝素存在于整个发育过程和成年期。虽然HA的功能可能是由HA结合蛋白介导的，但尚未有细胞或组织特异性HA结合蛋白的报道。为了表征中枢神经系统细胞外基质的组成，我们试图鉴定神经ha结合蛋白。我们在此报道了与ha结合蛋白的蛋白聚糖串联重复序列（PTR）家族成员序列高度同源的cDNA的分离和鉴定。与其他ha结合蛋白不同，这种cDNA的表达仅限于中枢神经系统。我们建议将该基因命名为BEHAB，即大脑富集透明质酸结合蛋白。BEHAB mRNA的表达受发育调控；在胚胎后期首次检测到表达，并在出生后的前两周达到峰值。在胚胎中，BEHAB在有丝分裂活跃的细胞中表达水平最高。BEHAB的序列在大鼠和猫之间有很长一段相同的序列，这表明编码的蛋白质在功能上很重要。BEHAB的大小和序列与它可能具有类似连接蛋白的功能，稳定HA和脑蛋白聚糖之间的相互作用的可能性一致。这些观察结果表明存在其他组织特异性ha结合蛋白。
Hyaluronan (HA) is a ubiquitous component of the extracellular matrix of all tissues. In the mammalian central nervous system (CNS) HA is present throughout development and into adulthood. While the functions of HA are likely to be mediated by HA-binding proteins, no cell or tissue specific HA-binding proteins have been reported. In an effort to characterize the composition of the extracellular matrix of the CNS, we sought to identify neural HA-binding proteins. We report here the isolation and characterization of a cDNA with a high degree of sequence homology to members of the proteoglycan tandem repeat (PTR) family of HA-binding proteins. Unlike other HA-binding proteins, the expression of this cDNA is restricted to the CNS. We propose the name BEHAB, Brain Enriched HyAluronan Binding protein, for this gene. The expression of BEHAB mRNA is developmentally regulated; expression is first detected in the late embryonic period and peaks during the first two postnatal weeks. In the embryo, BEHAB is expressed at highest levels in mitotically active cells. The sequence of BEHAB has long stretches of identity between rat and cat, suggesting that the encoded protein is functionally important. The size and sequence of BEHAB are consistent with the possibility that it could serve a function like link protein, stabilizing interactions between HA and brain proteoglycans. These observations suggest that existence of other tissue specific HA-binding proteins.