Resistance to state transitions in responsiveness is differentially modulated by different volatile anaesthetics in male mice
Resistance to state transitions in responsiveness is differentially modulated by different volatile anaesthetics in male mice
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DOI：
10.1016/j.bja.2020.05.031
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发表时间：
2020-09-01
影响因子：
9.8
通讯作者：
Proekt, Alex
中科院分区：
文献类型：
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作者：
Wasilczuk, Andrzej Z.;Harrison, Benjamin A.;Proekt, Alex
背景：最近的研究指出了基于人群和基于个体的麻醉药理学之间的根本区别。在群体水平上，麻醉效力被定义为药物浓度与对刺激反应的可能性之间的关系。在个体水平上，即使麻醉剂浓度保持恒定，也会观察到反应状态和无反应状态之间的波动。值得注意的是，这些自发波动表现出对状态转换R-st的抵抗力。因此，每个个体的响应概率不仅取决于药物浓度，还取决于对先前刺激的响应。在这里，我们假设Rst与药物效力不同，并且受不同麻醉剂的不同调节。在异氟烷的等效稳态浓度下，对（14-24周龄）C57 BL/6 J雄性小鼠（n=60）进行重复翻正反射（RR）测定结果：所有试验麻醉剂的RR均出现波动。对这些波动的分析表明，不同麻醉剂对R-st的调节差异显著（F[2，56.01]=49.59; P
Background: Recent studies point to a fundamental distinction between population-based and individual-based anaesthetic pharmacology. At the population level, anaesthetic potency is defined as the relationship between drug concentration and the likelihood of response to a stimulus. At the individual level, even when the anaesthetic concentration is held constant, fluctuations between the responsive and unresponsive states are observed. Notably, these spontaneous fluctuations exhibit resistance to state transitions R-st. Therefore, the response probability in each individual depends not just upon the drug concentration, but also upon responses to previous stimuli. Here, we hypothesise that Rst is distinct from drug potency and is differentially modulated by different anaesthetics.Methods: Adult (14-24 weeks old) C57BL/6J male mice (n=60) were subjected to repeated righting reflex (RR) assays at equipotent steady-state concentrations of isoflurane (0.6 vol%), sevoflurane (1.0 vol%), and halothane (0.4 vol%).Results: Fluctuations in RR were observed for all tested anaesthetics. Analysis of these fluctuations revealed that R-st was differentially modulated by different anaesthetics (F[2, 56.01]=49.59; P