Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stress tolerance
Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stress tolerance
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S-腺苷甲硫氨酸脱羧酶基因的下调导致植物长度、花粉活力和非生物胁迫耐受性降低
DOI：
10.1007/s11240-013-0405-0
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发表时间：
2014-03-01
影响因子：
3
通讯作者：
Lu, Shaoyun
中科院分区：
文献类型：
--
作者：
Chen, Miao;Chen, Jingjing;Lu, Shaoyun
腺苷甲硫氨酸脱羧酶（SAMDC）是合成多胺的关键酶，调节植物生长发育和逆境应答。通过RNA干扰获得下调OsSAMDC 2的转基因水稻植株。在转基因水稻中，OsSAMDC 2、OsSAMDC 1和OsSAMDC 4的转录水平均降低，同时沿着精脒（Spd）和精蛋白（Spm）水平以及多胺氧化酶活性降低。转基因株系表现出降低的顶端优势表型，具有较低的株长、较高的分蘖数、降低和延迟的种子萌发率、降低的花粉生活力、结实率和单株籽粒产量。在胁迫条件下，转基因植物对多种非生物胁迫（干旱、盐碱和低温）的耐受性也降低，这与抗氧化酶活性的抑制有关。我们的研究结果表明，Spd和Spm是必要的，以维持正常的植物生长，花粉活力，结实率，籽粒产量和胁迫耐受性的水稻。
S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme for synthesis of polyamines, regulating plant growth and development as well as plant stress responses. Transgenic rice plants down-regulating OsSAMDC2 were generated by RNA interference. Transcript levels of OsSAMDC2, OsSAMDC1 and OsSAMDC4 were all reduced in transgenic rice, along with decreased levels of sperimidine (Spd) and spermin (Spm) and polyamine oxidase activity. The transgenic lines showed a reduced apical dominance phenotype with lower plant length, higher tiller numbers, reduced and delayed seed germination rate, and decreased pollen viability, seed setting rate and grain yield per plant. Tolerance to multiple abiotic stresses (drought, salinity, and chilling) was also reduced in transgenic plants in association with inhibition of antioxidant enzyme activities under stressed conditions. Our results suggest that Spd and Spm are essential for maintenance of normal plant growth, pollen viability, seed setting rate, grain yield and stress tolerance in rice.