Three-dimensional growth of differentiating MC3T3-E1 pre-osteoblasts on porous titanium scaffolds
Three-dimensional growth of differentiating MC3T3-E1 pre-osteoblasts on porous titanium scaffolds
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DOI：
10.1016/j.biomaterials.2005.05.046
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发表时间：
2005-12-01
期刊：
影响因子：
14
通讯作者：
Tabrizian, M
中科院分区：
文献类型：
--
作者：
St-Pierre, JP;Gauthier, M;Tabrizian, M
本研究通过体外实验评估了一种新型粉末冶金工艺生产的三维多孔钛支架在骨工程应用中的潜力。使用小鼠MC3T3 - E1前成骨细胞，以镜面抛光钛作为参考材料，研究了平均孔径在336到557μm之间的钛支架上细胞的增殖（DNA含量）、分化（碱性磷酸酶活性和骨钙素释放）以及矿化（钙含量）过程。采用扫描电子显微镜对结果进行定性验证。细胞在所有材料上增殖，在第9天达到平台期，泡沫材料上的增殖率（每天123%到163%）显著高于参考材料（每天80%）。在增殖阶段之后，多孔支架上的碱性磷酸酶活性也显著升高。然而，在分化过程末期，抛光钛上的细胞表现出更高的骨钙素释放量，导致细胞外基质更早矿化。尽管如此，在实验期末，所有材料的钙含量相似。通过各种分析确定，多孔结构的平均孔径对细胞没有重大影响，仅影响增殖阶段。因此，微结构钛支架引导前成骨细胞的行为趋向于能够使细胞外基质矿化的成熟状态。（c）2005爱思唯尔有限公司。保留所有权利。
The present work assesses the potential of three-dimensional porous titanium scaffolds produced by a novel powder metallurgy process for applications in bone engineering through in vitro experimentation. Mouse MC3T3-E1 pre-osteoblasts were used to investigate the proliferation (DNA content), differentiation (alkaline phosphatase activity and osteocalcin release) and mineralisation (calcium content) processes of cells on titanium scaffolds with average pore sizes ranging from 336 to 557 Pin, using mirror-polished titanium as reference material. Scanning electron microscopy was employed to qualitatively corroborate the results. Cells proliferate on all materials before reaching a plateau at day 9, with proliferation rates being significantly higher on foams (ranging from 123 to 163 percent per day) than on the reference material (80% per day). Alkaline phosphatase activity is also significantly elevated on porous scaffolds following the proliferation stage. However, cells on polished titanium exhibit greater osteocalcin release toward the end of the differentiation process, resulting in earlier mineralisation of the extracellular matrix. Nevertheless, the calcium content is similar on all materials at the end of the experimental period. Average pore size of the porous structures does not have a major effect on cells as determined by the various analyses, affecting only the proliferation stage. Thus, the microstructured titanium scaffolds direct the behaviour of pre-osteoblasts toward a mature state capable of mineralising the extracellular matrix. (c) 2005 Elsevier Ltd. All rights reserved.