Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women: A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles
Decreased inhibin B secretion is associated with the monotropic FSH rise in older, ovulatory women: A study of serum and follicular fluid levels of dimeric inhibin A and B in spontaneous menstrual cycles
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DOI：
10.1210/jc.81.7.2742
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发表时间：
1996-07-01
影响因子：
5.8
通讯作者：
Soules, MR
中科院分区：
文献类型：
--
作者：
Klein, NA;Illingworth, PJ;Soules, MR
本研究旨在利用特异性的双位点elisa法比较正常老年和年轻排卵期妇女循环和卵泡液（FF）中二聚体抑制素A和B的浓度。方法：对40-45岁（n=10）和20-25岁（n=13）正常排卵的女性进行卵泡期的研究，包括每日采血、经阴道超声检查和显性卵泡抽吸。当优势卵泡平均直径达到16 mm或血清雌二醇（E(2)）大于或等于550 pmol/L时，肌肉注射1万IU hCG， 32小时后经阴道卵泡抽吸。分析血清和FF样本中的E(2)、FSH、抑制素A和b。每日激素水平采用方差分析比较，平均结果采用t检验比较。结果：老年妇女优势卵泡发育更快，符合hCG周期天数标准10.6+/-0.4 vs 14.5+/-1.0 p
This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A and B utilizing specific two-site ELISAs for these hormones in normal older and younger ovulatory women. Methods: Normally ovulating women age 40-45 (n=10) and 20-25 (n=13) were studied throughout the follicular phase with daily blood sampling, transvaginal ultrasound examinations, and dominant follicle aspiration. When the dominant follicle reached a mean diameter of 16 mm or serum estradiol (E(2)) was greater than or equal to 550 pmol/L, 10,000 IU of hCG was administered intramuscularly followed 32 hours later by transvaginal follicle aspiration. Serum and FF samples were analyzed for E(2), FSH, and inhibin A and B. Daily hormone levels were compared by ANOVA, and mean results were compared using t-tests. Results: Older women developed a dominant follicle sooner, meeting criteria for hCG cycle day 10.6+/-0.4 vs. 14.5+/-1.0 p