Decreased annexin I expression in prostatic adenocarcinoma and in high-grade prostatic intraepithelial neoplasia
Decreased annexin I expression in prostatic adenocarcinoma and in high-grade prostatic intraepithelial neoplasia
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DOI：
10.1111/j.1365-2559.2005.02300.x
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发表时间：
2005-12-01
期刊：
影响因子：
6.4
通讯作者：
Yang, XJ
中科院分区：
文献类型：
--
作者：
Patton, KT;Chen, HM;Yang, XJ
目的：分析膜联蛋白I在前列腺癌中的表达。膜联蛋白I是一个结构相关的钙和磷脂结合蛋白家族，参与信号转导、DNA复制、细胞增殖和细胞凋亡。方法与结果：采用免疫组织化学方法，对77例前列腺癌(Gleason评分6，N=40；Gleason评分7~8，N=27；Gleason评分9~10，N=10)和高级别前列腺上皮内瘤变(PIN，N=50)中Annexin I的表达进行了分析。肿瘤细胞膜联蛋白I的免疫反应性分为阴性(5%的细胞)、局灶性阳性(5%~25%的细胞)或阳性(25%的细胞)。在76%的高级别前列腺癌(P<；0.0001)和81%的前列腺癌(P<；0.0001)中，Annexin I的表达降低或缺失(P<；0.0001)。在所有较高级别(Gleason评分7~10)的前列腺癌中，Annexin I的表达仅局限性阳性或缺失。结论：Annexin I的表达与前列腺癌的组织学分级呈负相关。通过显示Annexin I在高级别PIN、中级和高级别PIN中表达的进行性丢失，我们的发现表明Annexin I表达的丢失发生在前列腺癌发生的早期，并在整个肿瘤进展过程中变得更加突出。膜联蛋白I的表达缺失可作为前列腺癌发生发展的有用标志物。
Aims: To analyse annexin I expression in prostatic carcinoma. Annexin I belongs to a family of structurally related calcium and phospholipid-binding proteins implicated in signal transduction, DNA replication, cell proliferation and apoptosis. The decreased expression of annexin I, II and VII proteins has been reported in different types of cancer.Methods and results: Using immunohistochemistry, we analysed annexin I expression in 77 cases of prostatic adenocarcinoma (Gleason score 6, N = 40; Gleason scores 7-8, N = 27; and Gleason scores 9-10, N = 10) and high-grade prostatic intraepithelial neoplasia (PIN, N = 50). Immunoreactivity of annexin I in tumour cells was evaluated as negative (< 5% of cells), focally positive (5-25% of cells) or positive (> 25% of cells). In contrast to positive staining in adjacent benign prostatic epithelium, annexin I expression was decreased (focally positive) in 76% of cases of high-grade PIN (P < 0.0001) and was decreased or absent in 81% of prostatic adenocarcinomas (P < 0.0001). Annexin I expression in all higher grade tumours (Gleason scores 7-10) was only focally positive or absent.Conclusions: Expression of annexin I inversely correlates with the increasing histological grade of prostatic adenocarcinoma. By showing a progressive loss of annexin I expression in high-grade PIN, intermediate-grade and high-grade cancer, our findings suggest that the loss of annexin I expression occurs early in prostatic tumorigenesis and becomes more prominent throughout tumour progression. The loss of expression of annexin I may serve as a useful marker of prostate cancer development and progression.