Porous zirconia/hydroxyapatite scaffolds for bone reconstruction
Porous zirconia/hydroxyapatite scaffolds for bone reconstruction
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DOI：
10.1016/j.dental.2012.09.001
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发表时间：
2012-12-01
期刊：
影响因子：
5
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
An, Sang-Hyun;Matsumoto, Takuya;Imazato, Satoshi
客观的。高度多孔的磷灰石生物陶瓷支架作为细胞粘附、增殖和分化促进骨再生的三维（3D）模板已被广泛研究。然而，它们的脆性限制了它们的临床应用，特别是对于大骨缺损。方法。为了解决使用 ZrO2/羟基磷灰石 (HAp) 复合材料可以提高多孔材料的机械性能和细胞相容性的假设，我们制造了具有不同 ZrO2/HAp 比例的 ZrO2/HAp 复合支架，并评估了它们的特性。此外，将含有骨髓间充质干细胞（BMSCs）的多孔ZrO2/HAp支架植入临界尺寸的骨缺损中6周，以评估该材料的骨组织重建效果。当ZrO2含量从50wt%增加到100wt%时，ZrO2/HAp支架的孔隙率可以从72%调节到91%，支架的抗压强度从2.5MPa增加到13.8MPa。与单独使用 ZrO2 制成的支架相比，ZrO2/HAp 支架中的细胞粘附和增殖大大改善。此外，体内研究表明，负载 BMSC 的 ZrO2/HAp 支架为 BMSC 存活提供了合适的 3D 环境，并增强了植入材料周围的骨再生。意义。因此，我们表明多孔 ZrO2/HAp 复合支架具有优异的机械性能和细胞/组织相容性，并且将成为实现治疗大骨缺损所需的骨重建和再生的有前途的基材。 (C) 2012 年牙科材料学会。由爱思唯尔有限公司出版。保留所有权利。
Objective. Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect.Methods. To address the hypothesis that using a ZrO2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/HAp composite scaffolds with different ZrO2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material.Results. The porosity of a ZrO2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material.Significance. We thus showed that a porous ZrO2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects. (C) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.