Pretranslational regulation of myoglobin gene expression.
Pretranslational regulation of myoglobin gene expression.
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DOI：
10.1152/ajpcell.1987.252.4.c450
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发表时间：
1987-04
期刊：
影响因子：
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通讯作者：
L. Underwood;R. Williams
中科院分区：
文献类型：
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作者：
L. Underwood;R. Williams
我们已经使用印迹杂交技术和一个特定的反义RNA探针，以确定是否在哺乳动物横纹肌肌红蛋白基因表达的变化是由于翻译前调控事件。我们观察到，肌红蛋白mRNA的表达水平约10倍和5倍，分别在成年兔的心脏和比目鱼肌（慢收缩，氧化，骨骼肌）比胫骨前肌（快收缩，糖酵解，骨骼肌）。此外，当胫骨前肌的氧化能力增加了21天的间接电刺激，运动调节模型，肌红蛋白mRNA含量增加了约15倍。我们的结论是，翻译前机制是重要的肌红蛋白基因的表达在哺乳动物肌肉的调节。
We have used blot hybridization techniques and a specific anti-sense RNA probe to determine whether variation in myoglobin gene expression among mammalian striated muscles is attributable to pretranslational regulatory events. We observed that myoglobin mRNA was expressed to approximately 10- and 5-fold greater levels, respectively, in cardiac and soleus (slow-twitch, oxidative, skeletal) muscles of adult rabbits than in tibialis anterior (fast-twitch, glycolytic, skeletal) muscles. Furthermore, when oxidative capacity of tibialis anterior muscles was increased by 21 days of indirect electrical stimulation, a model of exercise conditioning, myoglobin mRNA content increased approximately 15-fold. We conclude that pretranslational mechanisms are important in regulation of myoglobin gene expression in mammalian muscles.