STABLE INHERITANCE OF PLASMID R1 REQUIRES 2 DIFFERENT LOCI
STABLE INHERITANCE OF PLASMID R1 REQUIRES 2 DIFFERENT LOCI
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DOI：
10.1128/jb.161.1.292-298.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
MOLIN, S
中科院分区：
文献类型：
--
作者：
GERDES, K;LARSEN, JEL;MOLIN, S
来自质粒R1的最大EcoRI片段介导稳定性表型，这是确保该低拷贝数质粒的稳定遗传所需的。当共价连接到小的，不稳定的R1衍生物，该片段使质粒稳定的野生型R1质粒。一个遗传分析表明，两个独立的行动稳定功能编码的EcoRI片段，这两个都有可能的部分稳定的迷你R1质粒。这两个基因座位于片段的相对两端。通过将这些区域插入来自p15组的不相关的不稳定质粒中也获得了稳定。这两种功能之一在稳定这种外源复制子方面非常有效。除了稳定性表型，这些基因以等位基因特异性方式发挥不相容性。稳定性功能似乎不会严重干扰质粒的拷贝数。
The largest EcoRI fragment from plasmid R1 mediates a stability phenotype which is required to ensure the stable inheritance of this low-copy-number plasmid. When covalently linked to small, unstable R1 derivatives, this fragment makes the plasmids as stable as the wild-type R1 plasmid. A genetic analysis showed that two independently acting stabilization functions are encoded by this EcoRI fragment, both of which have the potential of partial stabilization of mini-R1 plasmids. The two loci are located at opposite ends of the fragment. Stabilization was also obtained by inserting these regions in unrelated, unstable plasmids from the p15 group. One of the two functions was very efficient in stabilizing such foreign replicons. Besides the stability phenotype, these genes exert incompatibility in an allele-specific manner. The stability functions do not seem to interfere seriously with the copy number of the plasmid.