Proprioceptive Opsin Functions in Drosophila Larval Locomotion
Proprioceptive Opsin Functions in Drosophila Larval Locomotion
复制标题
DOI：
10.1016/j.neuron.2018.02.028
复制
发表时间：
2018-04
期刊：
影响因子：
16.2
通讯作者：
D. Zanini;Diego Giraldo;B. Warren;Radoslaw Katana;M. Andrés;S. Reddy;Stephanie Pauls;Nicola Schwedhelm-Domeyer;Bart R. H. Geurten;M. Göpfert
中科院分区：
文献类型：
--
作者：
D. Zanini;Diego Giraldo;B. Warren;Radoslaw Katana;M. Andrés;S. Reddy;Stephanie Pauls;Nicola Schwedhelm-Domeyer;Bart R. H. Geurten;M. Göpfert
动物依靠本体感受器的机械感觉反馈来控制运动性身体运动。出乎意料的是，我们发现这种运动控制需要视觉光学。干扰果蝇激素NINAE或Rh6会损害幼虫的运动和身体收缩，而不依赖于光线和视力。视蛋白位于幼虫体内的脉络膜本体感受器中，定位于纤毛树突。视蛋白的丢失损害了机械诱发的本体感受器棘波和纤毛超微结构。在没有NINAE或Rh6的情况下，NOMPC纤毛本体感受器漏出的机械转导通道和纤毛不活动(IAV)通道部分消失。运动被证明需要本体感受器中的视蛋白，受体被发现表达视蛋白基因Rh7，此外还有紧张素E和Rh6。除了涉及opsins在运动控制中的作用外，本研究还记录了非纤毛横纹体opsins在纤毛组织中的作用，为视蛋白进化中的关键转变提供了一个模型，并表明横纹体opsins的结构作用先于它们用于光检测。
Animals rely on mechanosensory feedback from proprioceptors to control locomotory body movements. Unexpectedly, we found that this movement control requires visual opsins. Disrupting theDrosophilaopsins NINAE or Rh6 impaired larval locomotion and body contractions, independently of light and vision. Opsins were detected in chordotonal proprioceptors along the larval body, localizing to their ciliated dendrites. Loss of opsins impaired mechanically evoked proprioceptor spiking and cilium ultrastructure. Without NINAE or Rh6, NOMPC mechanotransduction channels leaked from proprioceptor cilia and ciliary Inactive (Iav) channels partly disappeared. Locomotion is shown to require opsins in proprioceptors, and the receptors are found to express the opsin geneRh7, in addition toninaEandRh6. Besides implicating opsins in movement control, this documents roles of non-ciliary, rhabdomeric opsins in cilium organization, providing a model for a key transition in opsin evolution and suggesting that structural roles of rhabdomeric opsins preceded their use for light detection.