Interaction among bovine somatotropin, insulin, and gonadotropins on steroid production by bovine granulosa and thecal cells.
Interaction among bovine somatotropin, insulin, and gonadotropins on steroid production by bovine granulosa and thecal cells.
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牛生长激素、胰岛素和促性腺激素之间的相互作用对牛颗粒和膜细胞类固醇产生的影响。
DOI：
10.3168/jds.s0022-0302(96)76429-9
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发表时间：
1996
影响因子：
3.5
通讯作者：
R. Stewart
中科院分区：
文献类型：
--
作者：
L. Spicer;R. Stewart
本研究的目的是确定 bST、胰岛素和促性腺激素之间的相互作用对牛卵泡颗粒和膜细胞产生类固醇的影响。小（1至5毫米）和大（>或= 8毫米）卵泡的颗粒细胞产生的雌二醇基础（表示为每24小时每10（5）个细胞的雌二醇皮克数）不受50或300 ng/ml bST的影响，但300 ng/ml的bST抑制由FSH加胰岛素在小和大细胞中诱导的雌二醇产生毛囊。 3 至 100 ng/ml 的 bST 不会影响大卵泡颗粒细胞的孕酮产生和增殖。在 LH 诱导的雄烯二酮产量增加 3 倍以上的鞘膜细胞培养物中，3 至 30 ng/ml 的 bST 进一步将雄烯二酮产量增加 29% 至 42%，但细胞增殖和孕酮产量不受 bST 影响。在 LH 诱导的雄烯二酮产量增加 < 2 倍的鞘膜细胞培养物中，3 至 30 ng/ml 的 bST 可抑制雄烯二酮产量 32% 至 33%，并抑制细胞增殖 9% 至 13%，但黄体酮不受 bST 影响。总之，只有药理学剂量的bST抑制颗粒细胞产生雌二醇，但生理剂量的bST改变了鞘细胞产生雄烯二酮，这表明bST可能在牛的颗粒细胞功能中没有重要作用，但可能在鞘细胞功能中发挥作用。
The objective of the present study was to determine the interactions among bST, insulin, and gonadotropins on steroid production by granulosa and thecal cells from bovine follicles. Basal production of estradiol by granulosa cells from small (1 to 5 mm) and large (> or = 8 mm) follicles (expressed as picograms of estradiol per 10(5) cells per 24 h) was not affected by 50 or 300 ng/ml of bST, but 300 ng/ml of bST inhibited estradiol production that was induced by FSH plus insulin in cells from small and large follicles. Progesterone production and proliferation by granulosa cells from large follicles were not affected by 3 to 100 ng/ml of bST. In cultures of thecal cells that exhibited a > 3-fold increase in androstenedione production induced by LH, 3 to 30 ng/ml of bST further increased androstenedione production by 29 to 42%, but cell proliferation and progesterone production were unaffected by bST. In cultures of thecal cells that exhibited a < 2-fold increase in androstenedione production induced by LH, 3 to 30 ng/ml of bST inhibited androstenedione production by 32 to 33% and inhibited cell proliferation by 9 to 13%, but progesterone was unaffected by bST. In summary, only pharmacologic doses of bST inhibited estradiol production by granulosa cells, but physiologic doses of bST altered androstenedione production by thecal cells, which indicated that bST might not have an important role in granulosa cell function but might play a role in thecal cell function in cattle.
影响因子：
4.8
作者：
ROBERTS, AJ;SKINNER, MK
通讯作者：
SKINNER, MK