Embryonic stem cell models in vascular biology
Embryonic stem cell models in vascular biology
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DOI：
10.1111/j.1538-7836.2009.03427.x
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发表时间：
2009-07-01
影响因子：
10.4
通讯作者：
Claesson-Welsh, L.
中科院分区：
文献类型：
--
作者：
Li, X.;Claesson-Welsh, L.
胚胎干细胞已经成为血管生物学中研究血管发生和血管生成细节的一种成熟工具。胚胎干细胞衍生的内皮细胞用于治疗也有未来的潜力。通过比较分化干细胞衍生的内皮细胞的特征及其对外部刺激的反应性与原代内皮细胞和体内模型的反应性来评估该模型是很重要的。通过对小鼠胚胎干细胞的培养，我们发现分化干细胞高度适应于分析不依赖于流动的生化和细胞生物学过程。内皮细胞的功能可以在体内胚胎致死性突变或缺失的情况下进行研究。分化干细胞中新生血管生成的许多特征（如果不是全部的话）与体内过程非常相似。
Embryonic stem cells have become an established tool in vascular biology to study the details of vasculogenesis as well as angiogenesis. There is also a future potential in using embryonic stem cell-derived endothelial cells for therapeutic purposes. It is important to evaluate this model by comparing features of endothelial cells derived from differentiating stem cells and their responsiveness to external stimuli to those of primary endothelial cells and to in vivo models. Through culture of mouse embryonic stem cell we discovered that differentiating stem cells are highly amenable to analyzing biochemical and cell biologic processes that are independent of flow. Endothelial cell function can be studied in the context of mutations or deletions that are embryonically lethal in vivo. Many, if not all, of the features of sprouting angiogenesis in differentiating stem cells closely mimic the in vivo process.