Cutting Edge: TLR13 Is a Receptor for Bacterial RNA
Cutting Edge: TLR13 Is a Receptor for Bacterial RNA
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DOI：
10.4049/jimmunol.1200898
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发表时间：
2012-09-15
影响因子：
4.4
通讯作者：
Dalpke, Alexander H.
中科院分区：
文献类型：
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作者：
Hidmark, Asa;von Saint Paul, Antonia;Dalpke, Alexander H.
细菌RNA （bRNA）可以通过一种未知的受体诱导巨噬细胞和树突状细胞（DCs）产生细胞因子。小鼠dc的基因表达分析表明，bRNA诱导的基因调控与R848刺激TLR7诱导的基因调控相似。虽然bRNA诱导细胞因子不需要TLR7，但需要tlr相关蛋白MyD88和UNC93B。TLR13是一种小鼠内体TLR，被描述为与UNC93B相互作用，迄今为止没有表征的配体。TLR13小干扰RNA可降低bRNA对dc细胞因子的诱导作用。此外，转染TLR13而不转染TLR7或8的中国仓鼠卵巢细胞能够以rna特异性的方式激活NF-kappa B，以响应bRNA或化脓性链球菌。此外，TLR7拮抗剂IRS661在TLR7缺失的情况下也能抑制TLR13信号通路，降低dc对整个革兰氏阳性菌的识别。结果确定TLR13是bRNA的受体。免疫学杂志，2012,19(9):2717-2721。
Bacterial RNA (bRNA) can induce cytokine production in macrophages and dendritic cells (DCs) through a previously unidentified receptor. Gene expression analysis of murine DCs showed that bRNA induced gene regulation similar to that induced by stimulation of TLR7 with R848. Although TLR7 was dispensable for cytokine induction by bRNA, TLR-associated proteins MyD88 and UNC93B were required. TLR13 is an endosomal murine TLR that has been described to interact with UNC93B with, so far, no characterized ligand. Small interfering RNA against TLR13 reduced cytokine induction by bRNA in DCs. Moreover, Chinese hamster ovary cells transfected with TLR13, but not with TLR7 or 8, could activate NF-kappa B in response to bRNA or Streptococcus pyogenes in an RNA-specific manner. TLR7 antagonist IRS661 could, in addition, inhibit TLR13 signaling and reduced recognition of whole Gram-positive bacteria by DCs, also in the absence of TLR7. The results identify TLR13 as a receptor for bRNA. The Journal of Immunology, 2012, 189: 2717-2721.