Evolution of Network Synchronization during Early Epileptogenesis Parallels Synaptic Circuit Alterations
Evolution of Network Synchronization during Early Epileptogenesis Parallels Synaptic Circuit Alterations
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DOI：
10.1523/jneurosci.4007-14.2015
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发表时间：
2015-07-08
影响因子：
5.3
通讯作者：
Staley, Kevin J.
中科院分区：
文献类型：
--
作者：
Lillis, Kyle P.;Wang, Zemin;Staley, Kevin J.
在继发性癫痫中，在脑损伤和自发性癫痫发作之间的潜伏期内，易癫痫神经网络进化。演变的性质在很大程度上是未知的，甚至其完整性在癫痫发作的开始最近受到挑战的措施，逐渐减少间隔之间的后续癫痫发作。神经元活动的顺序钙成像，在小鼠海马体外制剂的锥体细胞层，在早期创伤后癫痫表现出快速增加的神经元参与发作间期活动的分数。随后是更渐进的增加，在其中个别神经元加入每个发展中的癫痫发作，神经元活动的成对相关性作为一个函数的距离分开的对，和网络范围内的功能连接的措施。这些数据支持癫痫网络中突触连接在潜伏期之后的持续演变：早期癫痫发作发生在复发性兴奋性通路主要是多突触的时候，而癫痫发作后持续的突触重塑增强了网络内的连接以及癫痫发作活动的发作和扩散。
In secondary epilepsy, a seizure-prone neural network evolves during the latent period between brain injury and the onset of spontaneous seizures. The nature of the evolution is largely unknown, and even its completeness at the onset of seizures has recently been challenged by measures of gradually decreasing intervals between subsequent seizures. Sequential calcium imaging of neuronal activity, in the pyramidal cell layer of mouse hippocampal in vitro preparations, during early post-traumatic epileptogenesis demonstrated rapid increases in the fraction of neurons that participate in interictal activity. This was followed by more gradual increases in the rate at which individual neurons join each developing seizure, the pairwise correlation of neuronal activities as a function of the distance separating the pair, and network-wide measures of functional connectivity. These data support the continued evolution of synaptic connectivity in epileptic networks beyond the latent period: early seizures occur when recurrent excitatory pathways are largely polysynaptic, while ongoing synaptic remodeling after the onset of epilepsy enhances intranetwork connectivity as well as the onset and spread of seizure activity.