Mussel-inspired polyurethane coating for bio-surface functionalization to enhance substrate adhesion and cell biocompatibility
Mussel-inspired polyurethane coating for bio-surface functionalization to enhance substrate adhesion and cell biocompatibility
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受贻贝启发的聚氨酯涂层，用于生物表面功能化，以增强基材粘附性和细胞生物相容性
DOI：
10.1080/09205063.2022.2085342
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Qiang Fu
中科院分区：
文献类型：
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作者：
Hao Ming;ChenXu Tian;Nan He;Xin Zhao;Feng Luo;Zhen Li;Jiehua Li;Hong Tan;Qiang Fu
由于组织相容性差，相当多的植入材料容易引起严重的炎症反应，从而导致各种并发症和植入失败。对这些植入材料进行表面涂层改性是解决这一问题的重要技术之一。然而，制造具有足够的亲水性和优异的生物相容性的涂层仍然是一个挑战。受贻贝附着机理的启发，以二异氰酸酯为硬段，聚四亚甲基醚-乙二醇为软段，赖氨酸-多巴胺（LDA）和丁二醇为扩链剂，采用逐步聚合法合成了一系列贻贝聚氨酯（PU-LDAs），并将其应用于不锈钢、玻璃和PP使用一个简单的一步涂布工艺。通过180°剥离试验表明，3，4-二羟基苯丙氨酸（DOPA）基团的引入可显著提高涂层与基材的附着力。含高LDA含量的PU-LDA 100涂层在不锈钢、玻璃和PP表面的剥离强度分别为76.3、48.5和67.5 N/m，比不含LDA的PU-LDA 00涂层提高了106.2%、246.4%和192.2%。同时，该聚氨酯涂层具有较低的免疫炎症反应，为植入材料的表面改性提供了一种通用的方法。此外，PU-LDAs中的DOPA基团可以与细胞膜表面的氨基和巯基联合收割机结合，从而改善细胞的粘附和生长。因此，它在生物医用植入材料领域具有很大的潜在应用价值。
Considerable implant materials are prone to cause a severe inflammatory reaction due to poor histocompatibility, which leads to various complications and implant failure. Surface coating modification of these implant materials is one of the most important techniques to settle this problem. However, fabricating a coating with both adequate adhesiveness and excellent biocompatibility remains a challenge. Inspired by the adhesion mechanism of mussels, a series of mussel-inspired polyurethanes (PU-LDAs) were synthysized through a step growth polymerization based on hexamethylene diisocyanate as a hard segment, polytetra-methylene-ether-glycol as a soft segment, lysine-dopamine (LDA) and butanediol as chain extenders with different mole ratios.The coatings of PU-LDAs were applied to various substrates, such as stainless steel, glass and PP using a facile one-step coating process. The introduction of 3,4-dihydroxyphenylalanine (DOPA) groups can greatly improve the adhesion ability of the coatings to the substrates demonstrated by a 180° peel test. The peel strength of the PU-LDA100 coating containing high LDA content was 76.3, 48.5 and 67.5 N/m, which was 106.2%, 246.4% and 192.2% higher than that of the PU-LDA00 coating without LDA on the surface of stainless steel, glass and PP, respectively. Meanwhile, this PU coating has a lower immune inflammatory response which provides a universal method for surface modification of implant materials. Moreover, the DOPA groups in PU-LDAs could combine with the amino and thiol groups on cell membrane surface, leading to the improvement of cell adhesion and growth. Therefore, it has great potential application in the field of biomedical implant materials for the clinic.