Involvement of α-galactosidase OmAGAL2 in planteose hydrolysis during seed germination of Orobanche minor.
Involvement of α-galactosidase OmAGAL2 in planteose hydrolysis during seed germination of Orobanche minor.
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DOI：
10.1093/jxb/erab527
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发表时间：
2022-04-05
影响因子：
6.9
通讯作者：
Ohta D
中科院分区：
文献类型：
--
作者：
Okazawa A;Baba A;Okano H;Tokunaga T;Nakaue T;Ogawa T;Shimma S;Sugimoto Y;Ohta D
列当种子中储存在胚周围组织中的植物糖在萌发过程中在胚周围的质外体中被α-半乳糖苷酶OmAGAL 2水解。列当科（Orobanchaceae）的根寄生杂草，如独脚金（独脚金属（Striga spp.）和肉苁蓉（列当属和蒲葵属），在世界范围内造成严重的农业损失，并且已经努力控制这些寄生杂草。了解根寄生杂草的生命周期中的特征生理过程对于确定生长调节剂的特定目标特别重要。在我们以前的研究中，发现植物糖代谢在感受到独脚金内酯后很快被激活。minor.野尻霉素抑制植物糖代谢，阻碍种子萌发。轻微，表明根寄生杂草控制的可能目标。本文研究了车前草干种子中车前草糖的分布。通过基质辅助激光解吸/电离-质谱成像检测微量元素。在胚胎周围的组织中检测到了植物糖，但在胚胎内部没有，这支持了植物糖作为储存碳水化合物的作用。α-半乳糖苷酶家族成员OmAGAL 2的生化测定和分子表征表明，该酶在感受独脚金内酯后参与胚周围质外体中的植物糖水解，为胚提供萌发所必需的己糖。这些结果表明OmAGAL 2是一个潜在的根寄生杂草控制的分子靶标。
Planteose stored in tissues surrounding the embryo in Orobanche minordry seeds is hydrolysed in the apoplast around the embryo by α-galactosidase OmAGAL2 during germination. Root parasitic weeds of the Orobanchaceae, such as witchweeds (Striga spp.) and broomrapes (Orobanche and Phelipanche spp.), cause serious losses in agriculture worldwide, and efforts have been made to control these parasitic weeds. Understanding the characteristic physiological processes in the life cycle of root parasitic weeds is particularly important to identify specific targets for growth modulators. In our previous study, planteose metabolism was revealed to be activated soon after the perception of strigolactones in germinating seeds of O. minor. Nojirimycin inhibited planteose metabolism and impeded seed germination of O. minor, indicating a possible target for root parasitic weed control. In the present study, we investigated the distribution of planteose in dry seeds of O. minor by matrix-assisted laser desorption/ionization–mass spectrometry imaging. Planteose was detected in tissues surrounding—but not within—the embryo, supporting its suggested role as a storage carbohydrate. Biochemical assays and molecular characterization of an α-galactosidase family member, OmAGAL2, indicated that the enzyme is involved in planteose hydrolysis in the apoplast around the embryo after the perception of strigolactones, to provide the embryo with essential hexoses for germination. These results indicate that OmAGAL2 is a potential molecular target for root parasitic weed control.
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