Metaproteomics: Sample Preparation and Methodological Considerations
Metaproteomics: Sample Preparation and Methodological Considerations
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DOI：
10.1007/978-3-030-12298-0_8
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Arntzen, Magnus O.
中科院分区：
文献类型：
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作者：
Kunath, Benoit J.;Minniti, Giusi;Arntzen, Magnus O.
元组学技术在过去的十年中发展迅速，并经常用于微生物生态学研究微生物在其自然生态系统中独立于培养限制。元蛋白质组学与宏基因组学相结合，使得能够定量评估来自财团的个体成员的表达蛋白和途径。总之，元蛋白质组学和元基因组学可以详细了解哪些生物体占据特定的代谢生态位，它们如何相互作用，以及它们如何利用营养物质，这些见解可以直接从环境样本中获得。在这里，我们概述了成功的定量元蛋白质组学分析所需的样品制备，数据库生成和其他方法学考虑的关键方面，我们描述了与宏基因组学集成增强功能输出的案例研究。
Meta-omic techniques have progressed rapidly in the past decade and are frequently used in microbial ecology to study microorganisms in their natural ecosystems independent from culture restrictions. Metaproteomics, in combination with metagenomics, enables quantitative assessment of expressed proteins and pathways from individual members of the consortium. Together, metaproteomics and metagenomics can provide a detailed understanding of which organisms occupy specific metabolic niches, how they interact, and how they utilize nutrients, and these insights can be obtained directly from environmental samples. Here, we outline key aspects of sample preparation, database generation, and other methodological considerations that are required for successful quantitative metaproteomic analyses and we describe case studies on the integration with metagenomics for enhanced functional output.