Immunocytochemical localization of parathyroid hormone-like peptide in the rat fetus.
Immunocytochemical localization of parathyroid hormone-like peptide in the rat fetus.
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发表时间：
1991-12
期刊：
影响因子：
11.2
通讯作者：
R. Campos;S. Asa;D. Drucker
中科院分区：
文献类型：
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作者：
R. Campos;S. Asa;D. Drucker
甲状旁腺素样肽（PLP）的研究表明，PLP基因的表达是诱导血清，生长因子和放线菌酮。在诱导细胞分化后也观察到PLP基因表达的快速诱导。PLP基因表达的这些特征与PLP在调节细胞生长和分化中的作用一致。为了了解PLP在发育细胞和组织中的生物学特性，我们研究了PLP基因在胚胎和新生大鼠中的表达分布。在第14天的胎鼠中，PLP通过免疫细胞化学定位于皮肤、血管平滑肌、骨骼肌、心脏、肝脏、肾脏、肺和胃肠道。到第18天，在胎儿垂体和肾上腺髓质以及内分泌胰腺中也检测到PLP免疫阳性。尽管在第14天的脑中检测到很少的PLP免疫阳性细胞，但在第18天的脑中，在诸如脉络丛的区域中，PLP免疫阳性的分散区域是明显的。在第14天为阳性的第18天组织中也检测到PLP的免疫染色。胎儿睾丸中的染色模式，其中PLP强烈定位于曲细精管，不同于成人睾丸，其中PLP主要定位于间质细胞。PLP定位于胎肝的肝细胞，但不定位于造血成分。新生肝细胞呈弱PLP免疫阳性，成年大鼠肝脏中未检测到PLP。北方印迹分析表明，在胎儿脑，肝，心脏，肺和肠中存在单一的1.4-磷酸酶PLP mRNA转录本。这些研究的结果表明，PLP基因广泛表达在不同数量的胎鼠组织。PLP免疫阳性的细胞和组织定位在从胎儿到新生儿和成人组织的过渡中保持相当恒定，除了在睾丸中发生PLP产生细胞的细胞开关和肝脏中发生PLP基因表达在出生后逐渐消失。
Studies of parathyroid hormone-like peptide (PLP) have demonstrated that PLP gene expression is inducible by serum, growth factors, and cycloheximide. Rapid induction of PLP gene expression has also been observed following the induction of cell differentiation. These features of PLP gene expression are consistent with a role for PLP in the regulation of cell growth and differentiation. To understand the biology of PLP in developing cells and tissues, we have studied the distribution of PLP gene expression in the fetal and neonatal rat. PLP was localized by immunocytochemistry to skin, vascular smooth muscle, skeletal muscle, heart, liver, kidney, lung, and gastrointestinal tract in Day 14 fetal rat. By Day 18 PLP immunopositivity was also detected in both fetal pituitary and adrenal medulla, as well as in endocrine pancreas. Whereas few PLP-immunopositive cells were detected in Day 14 brain, scattered areas of PLP immunopositivity were evident in Day 18 brain, in regions such as the choroid plexus. Immunostaining for PLP was also detected in Day 18 tissues that were positive on Day 14. The pattern of staining in fetal testis, where PLP was strongly localized to seminiferous tubules, differed from adult testis, where PLP was localized predominantly in Leydig cells. PLP was localized to the hepatocytes but not to the hematopoietic elements in fetal liver. Neonatal hepatocytes were weakly PLP immunopositive, and PLP was not detected in adult rat liver. Northern blot analysis demonstrated the presence of a single 1.4-kilobase PLP mRNA transcript in fetal brain, liver, heart, lung, and intestine. The results of these studies demonstrate that the PLP gene is widely expressed in a diverse number of fetal rat tissues. The cellular and tissue localization of PLP immunopositivity remains fairly constant in the transition from fetal to neonatal and adult tissues except in the testis, where a cellular switch in PLP-producing cells occurs, and the liver, where PLP gene expression is progressively extinguished postnatally.