A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.
A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.
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DOI：
10.1016/j.atherosclerosis.2008.07.001
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发表时间：
2009-03
期刊：
影响因子：
5.3
通讯作者：
Formato, Marilena
中科院分区：
文献类型：
--
作者：
Lepedda, Antonio J.;Cigliano, Antonio;Cherchi, Gian Mario;Spirito, Rita;Maggioni, Marco;Carta, Franco;Turrini, Franco;Edelstein, Celina;Scanu, Angelo M.;Formato, Marilena
通过使用蛋白质组学，我们分离并鉴定了在稳定和不稳定的人颈动脉粥样硬化斑块中表达/保留的蛋白质。斑块不稳定的标准是存在覆盖泡沫或坏死核心的薄纤维帽或裂缝帽，以及存在明显的出血性、溃疡性或血栓性斑块。从动脉内膜切除术标本（19个稳定斑块，29个不稳定斑块）中提取蛋白质，并通过二维凝胶电泳分离。使用PD-Quest软件分析考马斯蓝染色的凝胶。通过基质辅助激光解吸/电离质谱法使用NCBI数据库鉴定了对应于33种不同蛋白质的总共57个不同的斑点。大多数斑点存在于两种类型的提取物中，尽管它们之间在丰度上存在显著差异（p<0.05）。与稳定的斑块相比，不稳定的斑块显示出保护酶SOD 3和GST、小的热休克蛋白HSP 27和HSP 20、膜联蛋白A10和Rho GDI的丰度降低。在不稳定斑块中含量较高的蛋白质为：铁蛋白轻亚基、SOD 2和纤维蛋白原片段D.对于纤维蛋白原片段D，通过蛋白质印迹分析证实了不稳定斑块与稳定斑块中的水平增加。由于已知许多差异表达的蛋白质在炎症和氧化应激中具有功能性作用，我们推测它们可能参与与斑块稳定性相关的事件。
By using proteomics we isolated and identified proteins that were expressed/retained in stable and unstable human carotid artery atherosclerotic plaques. The criteria for plaque instability were the presence of a thin fibrous cap or fissured cap covering the foamy or necrotic core, and the presence of overt, hemorrhagic, ulcerated or thrombotic plaques. Proteins were extracted from finely minced endarterectomy specimens (19 stable, 29 unstable plaques) and separated by 2-dimensional gel electrophoresis. Coomassie Blue-stained gels were analysed using PD-Quest software. A total of 57 distinct spots corresponding to 33 different proteins were identified by matrix assisted laser desorption/ionization mass spectrometry using the NCBI database. Most of the spots were present in both types of extracts, although significantly (p<0.05) differing in abundance between them. Compared to stable plaque, unstable ones showed reduced abundance of: protective enzymes SOD3 and GST, small heat shock proteins HSP27 and HSP20, annexin A10, and Rho GDI. In unstable plaques the more abundant proteins were: ferritin light subunit, SOD 2 and fibrinogen fragment D. For fibrinogen fragment D, the increased levels in unstable versus stable plaques was confirmed by Western blot analysis. Since many of the differentially expressed proteins are known to have a functional role in inflammation and oxidative stress, we speculate that they may be involved in events relating to plaque stability.
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DOI：
10.1161/01.atv.0000104013.71118.53
发表时间：
2004-01-01
影响因子：
8.7
作者：
Formato, M;Farina, M;Scanu, AM
通讯作者：
Scanu, AM
影响因子：
8.7
作者：
Luoma, JS;Strålin, P;Ylä-Herttuala, S
通讯作者：
Ylä-Herttuala, S
DOI：
10.1161/01.atv.0000220108.97208.67
发表时间：
2006-06-01
影响因子：
8.7
作者：
Martin-Ventura, Jose Luis;Nicolas, Valentin;Meilhac, Olivier
通讯作者：
Meilhac, Olivier
动脉粥样硬化的炎症。
DOI：
10.1161/atvbaha.108.179705
发表时间：
2012-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Libby P
通讯作者：
Libby P
影响因子：
7
作者：
Fach, EM;Garulacan, LA;Opiteck, GJ
通讯作者：
Opiteck, GJ