QTLs for resistance to the false brome rust Puccinia brachypodii in the model grass Brachypodium distachyon L.
QTLs for resistance to the false brome rust Puccinia brachypodii in the model grass Brachypodium distachyon L.
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DOI：
10.1139/g2012-001
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发表时间：
2012-02-01
期刊：
影响因子：
3.1
通讯作者：
Pecchioni, Nicola
中科院分区：
文献类型：
--
作者：
Barbieri, Mirko;Marcel, Thierry C.;Pecchioni, Nicola
模式草Brachypodium disachyon L.(Brachypodium)在研究草类与病原菌相互作用方面的潜力仍未得到充分开发。我们的目标是在短柄锈菌中鉴定与数量抗性相关的基因组区域。以抗性不同的自交系Bd3-1和Bd1-1为材料进行杂交，形成F-2群体。在苗期和生长后期对一株致病的短脚拟青霉的反应进行了评估。为了验证在F-2上获得的结果，在两个实验中对F-2衍生的F-3家族的抗性进行了量化。病情评估表明，抗性的数量和超越性分离。利用SSR和SNP标记构建了一个长812 cM、203个座位的AFLP连锁图谱，并将其定位于基因组序列。在第2、3、4号染色体上定位到3个抗雀麦锈病QTL，并通过试验检测到它们。本研究是青冈属植物的首次数量性状分析。对青枯病的抗性由几个QTL控制：两个作用于苗期，一个作用于苗期和生长后期。这些结果为阐明条锈菌数量抗性的分子基础提供了前景。
The potential of the model grass Brachypodium distachyon L. (Brachypodium) for studying grass-pathogen interactions is still underexploited. We aimed to identify genomic regions in Brachypodium associated with quantitative resistance to the false brome rust fungus Puccinia brachypodii. The inbred lines Bd3-1 and Bd1-1, differing in their level of resistance to P. brachypodii, were crossed to develop an F-2 population. This was evaluated for reaction to a virulent isolate of P. brachypodii at both the seedling and advanced growth stages. To validate the results obtained on the F-2, resistance was quantified in F-2-derived F-3 families in two experiments. Disease evaluations showed quantitative and transgressive segregation for resistance. A new AFLP-based Brachypodium linkage map consisting of 203 loci and spanning 812 cM was developed and anchored to the genome sequence with SSR and SNP markers. Three false brome rust resistance QTLs were identified on chromosomes 2, 3, and 4, and they were detected across experiments. This study is the first quantitative trait analysis in Brachypodium. Resistance to P. brachypodii was governed by a few QTLs: two acting at the seedling stage and one acting at both seedling and advanced growth stages. The results obtained offer perspectives to elucidate the molecular basis of quantitative resistance to rust fungi.