Rapid Identification of Candidate Genes for Seed Weight Using the SLAF-Seq Method in Brassica napus.
Rapid Identification of Candidate Genes for Seed Weight Using the SLAF-Seq Method in Brassica napus.
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DOI：
10.1371/journal.pone.0147580
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wei W
中科院分区：
文献类型：
--
作者：
Geng X;Jiang C;Yang J;Wang L;Wu X;Wei W
种子重量是油料作物种子产量的一个关键且直接的性状。了解其遗传机制对油菜（Brassica napus）育种中的产量提高具有重要意义。通过小孢子培养从大粒品系G - 42和小粒品系7 - 9的杂交后代中培育出250个双单倍体系。根据千粒重（TSW）数据，分别选取最重的46个品系和最轻的47个品系的个体DNA来构建两个混合DNA池。一种新的高通量测序技术，特异位点扩增片段测序（SLAF - seq），被用于在结合混合分组分析（BSA）的关联分析中鉴定TSW的候选基因。共开发了1933个高质量的多态性SLAF标记，并获得了4个与TSW相关的标记。在A09染色体上核苷酸25401885 - 25985931处约0.58 Mb的一个热点区域，包含91个候选基因，被确定与TSW性状紧密相关。从注释信息来看，四个基因（GSBRNA2T00037136001、GSBRNA2T00037157001、GSBRNA2T00037129001和GSBRNA2T00069389001）可能是与种子重量高度相关的有趣候选基因。
Seed weight is a critical and direct trait for oilseed crop seed yield. Understanding its genetic mechanism is of great importance for yield improvement in Brassica napus breeding. Two hundred and fifty doubled haploid lines derived by microspore culture were developed from a cross between a large-seed line G-42 and a small-seed line 7–9. According to the 1000-seed weight (TSW) data, the individual DNA of the heaviest 46 lines and the lightest 47 lines were respectively selected to establish two bulked DNA pools. A new high-throughput sequencing technology, Specific Locus Amplified Fragment Sequencing (SLAF-seq), was used to identify candidate genes of TSW in association analysis combined with bulked segregant analysis (BSA). A total of 1,933 high quality polymorphic SLAF markers were developed and 4 associated markers of TSW were procured. A hot region of ~0.58 Mb at nucleotides 25,401,885–25,985,931 on ChrA09 containing 91 candidate genes was identified as tightly associated with the TSW trait. From annotation information, four genes (GSBRNA2T00037136001, GSBRNA2T00037157001, GSBRNA2T00037129001 and GSBRNA2T00069389001) might be interesting candidate genes that are highly related to seed weight.