3-DIMENSIONAL MORPHOLOGY OF ASTROCYTES AND OLIGODENDROCYTES IN THE INTACT MOUSE OPTIC-NERVE
3-DIMENSIONAL MORPHOLOGY OF ASTROCYTES AND OLIGODENDROCYTES IN THE INTACT MOUSE OPTIC-NERVE
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DOI：
10.1007/bf01184071
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发表时间：
1994-08-01
期刊：
影响因子：
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通讯作者：
BERRY, M
中科院分区：
文献类型：
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作者：
BUTT, AM;COLQUHOUN, K;BERRY, M
采用激光扫描共聚焦显微镜和单细胞透明成像图，分别用裂解罗丹明葡聚糖染色和山萝卜过氧化物酶染色，对离体完整成熟小鼠视神经星形胶质细胞和少突胶质细胞的三维形态进行了分析。这些技术使单个染料填充细胞的整个过程场在所有平面上可视化，并解决了胶质细胞形态的精细细节。形态计量学分析表明，所有星形胶质细胞突起均有分支，分支结束于枕状表面、母血管上，并在神经内自由分布；在随附的论文中描述了以神经为终点的分支以兰维耶结为终点。星形胶质细胞被分类为一个单一的形态群体，其中每个细胞都具有多种功能。本研究的结果不支持星形胶质细胞可以细分为形态和功能亚型，即1型和2型，其过程分别结束于胶质细胞界限或节点的观点。对少突胶质细胞单位（定义为少突胶质细胞、其突和其所包裹的轴突）的三维分析显示，平均为19个轴突（范围12-35）提供单个髓鞘节段，平均节间长度为138 μ m（范围50-350 μ m）。小鼠视神经少突胶质细胞是一个均匀的群体，与大鼠视神经中的细胞明显相似。我们对少突胶质细胞形态的分析结果与单个少突胶质细胞支持的髓鞘的数量和节间长度与被鞘轴突的直径有关的观点一致。
The three-dimensional morphology of astrocytes and oligodendrocytes was analysed in the isolated intact mature mouse optic nerve, by correlating laser scanning confocal microscopy and camera lucida drawings of single cells, dye-filled with lysinated rhodamine dextran or horseradish peroxidase, respectively. These techniques enabled the entire process field of single dye-filled cells to be visualized in all planes and resolved the fine details of glial morphology. Morphometric analysis showed that the processes of ail astrocytes had branches ending at the pial surface, on brood vessels, and freely in the nerve; branches ending in the nerve were described to end at nodes of Ranvier in the accompanying paper. Astrocytes were classified into a single morphological population in which each cell subserved multiple functions. The results of this study do not support the contention that astrocytes can be subdivided into two morphological and functional subtypes, namely type-1 and type-2, which have processes ending either at the glia limitans or at nodes, respectively. Three-dimensional analysis of oligodendrocyte units, defined as the oligodendrocyte, its processes and the axons it ensheaths, showed the provision of single myelin segments for an average of 19 nearby axons (range 12-35) with a mean internodal length of 138 mu m (range 50-350 mu m). Mouse optic nerve oligodendrocytes were a homogeneous population and were markedly similar to those in the rat optic nerve. The results of our analysis of oligodendrocyte morphology are consistent with the view that the number and internodal length of myelin sheaths supported by a single oligodendrocyte are related to the diameter of the ensheathed axons.