Neutrophil traction stresses are concentrated in the uropod during migration
Neutrophil traction stresses are concentrated in the uropod during migration
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DOI：
10.1529/biophysj.106.102822
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发表时间：
2007-04-01
影响因子：
3.4
通讯作者：
Hammer, Daniel A.
中科院分区：
文献类型：
--
作者：
Smith, Lee A.;Aranda-Espinoza, Helim;Hammer, Daniel A.
我们发现，与强粘附、缓慢移动的细胞（如成纤维细胞）相比，中性粒细胞主要在细胞后部（尾足）施加相对于运动方向的收缩应力。而不是前缘拉动细胞，后方锚定细胞和收缩力集中的区域。这些牵引力在转弯时以秒到分钟的时间尺度迅速重新定位，它们的重新定位先于并设定了转弯时的运动方向。我们发现在趋化过程中，总平均均方根牵引力为28 +/- 10 nN，趋化过程中为67 +/- 10 nN。我们假设中性粒细胞后部的收缩力不仅破坏了尿足的粘附接触，而且还产生了向后挤压的收缩力，正如在变形虫或变形虫样细胞中看到的那样，细胞内形成了气泡，导致细胞内物质流向流体样板足。我们的发现提出了一种全新的中性粒细胞运动模式。
We find that in contrast to strongly adherent, slow moving cells such as fibroblasts, neutrophils exert contractile stresses largely in the rear of the cell ( uropod) relative to the direction of motion. Rather than the leading edge pulling the cell, the rear is both anchoring the cell and the area in which the contractile forces are concentrated. These tractions rapidly reorient themselves during a turn, on a timescale of seconds to minutes, and their repositioning precedes and sets the direction of motion during a turn. We find the total average rootmean-squared traction force to be 28 +/- 10 nN during chemokinesis, and 67 +/- 10 nN during chemotaxis. We hypothesize that the contraction forces in the back of the neutrophil not only break uropodial adhesive contacts but also create a rearward squeezing contractility, as seen in amoeboid or amoeboidlike cells and the formation of blebs in cells, causing a flow of intracellular material to the fluidlike lamellipod. Our findings suggest an entirely new model of neutrophil locomotion.