Relaxin in porcine corpora lutea during pregnancy and after hysterectomy.
Relaxin in porcine corpora lutea during pregnancy and after hysterectomy.
复制标题
怀孕期间和子宫切除术后猪黄体中的松弛素。
DOI：
10.1152/ajplegacy.1973.225.5.1215
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
D. F. Cox
中科院分区：
文献类型：
--
作者：
L. Anderson;J. Ford;R. Melampy;D. F. Cox
安德森，LL，JJ福特，RM MELAMPY和DF考克斯。松弛素在妊娠期和子宫切除术后猪黄体中的表达。Am. J. Physiol.225（5）：1215-1219. 1973.-通过测量小鼠耻骨间韧带的形成来测定猪黄体中的松弛素活性。松弛素水平保持低（例如，l-3 p&g）在整个发情周期。在交配的猪中，松弛素的水平在第20-29天增加四倍，并且在第10 -115天继续稳定地增加到峰值（> 300 pg/g）。在16小时内分娩激素水平下降到低值（如3 - 12/e/g），并保持低水平在第一个2周的哺乳期。在切除卵巢的猪中，黄体维持超过128天，松弛素水平在第110天稳定增加至最大值（例如，250 pg/g），然后在第128天下降（例如，50 pg/g），即使黄体仍然很大。通过在发情周期的黄体期注射雌激素，黄体维持超过100天;这些黄体中的松弛素水平在第128天开始下降，其方式类似于切除卵巢的猪。这些结果表明，松弛素水平可能是猪黄体老化过程中的一个指标。发情周期猪的黄体是松弛素的丰富来源，特别是在妊娠后期（I，7）。最近的证据表明，黄体组织中松弛素的水平在妊娠第105-110天达到最高，但在分娩前16小时内，它们下降到极低的值（2）。这些动物卵巢静脉血中的松弛素水平在第105-110天期间较低，在分娩前显著增加，然后在分娩后几小时（11 - 6小时）内降至极低值。这些结果表明，猪黄体积累和分泌松弛素在这些怀孕后期阶段。此外，随着妊娠期间松弛素水平的增加，细胞质颗粒在黄体细胞中积累，然后这些致密颗粒在分娩前消失;松弛素水平下降的时候。这些结果被解释为表明颗粒是松弛素储存的场所。
ANDERSON, LL, JJ FORD, RM MELAMPY, AND DF Cox. Relaxin in porcine corpora lutea during pegnancy and after hysterectomy. Am. J. Physiol. 225 (5): 1215-1219. 1973.-Relaxin activity in porcine corpora lutea was determined by measurement of interpubic ligament formation in mice. Relaxin levels remain low (eg, l-3 p&g) throughout the estrous cycle. In mated pigs the levels of relaxin increase fourfold by &ys 20-29 and continue to increase steadily to peak values (> 300 pg/g) by days X10-115. Within 16 hr of parturition hormone levels drop to low values (eg 3 12/e/g) and remain low during the first 2 weeks of lactation. In hysterectomized pigs the corpora lutea are maintained beyond 128 days, and relaxin levels increase steadily to maximal values (eg, 250 pg/g) by day II0 and then decrease(eg, 50 pg/g) by day 128 even though the corpora remain large. Corpora Iutea are maintained beyond 100 days by injecting estrogen during the luteal phase of the estrous cycle; relaxin levels in these corpora begin to decline by day 128 in a manner similar to that found in hysterectomized pigs. These results suggest that relaxin levels may be an indicator of the aging process in the porcine corpus luteum. estrous cycleTHE CORPUS LUTEUM of the pig is a rich source of relaxin, particularly during the late stages of pregnancy(I, 7). Recent evidence indicates that luteal tissue levels of relaxin are maximal at days 105-110 of pregnancy, but within 16 hr before parturition they decline to extremely low values (2). Relaxin levels in the ovarian venous blood in these same animals are low during days 105-110, increase markedly before parturition, and then drop to extremely low values within a few hours (ll 6 hr) of delivery. These results suggest that the porcine corpus luteum accumulates and secretes relaxin during these late stages of pregnancy. Furthermore, cytoplasmic granules accumulate in the luteal cells as the levels of relaxin increase during pregnancy, and then these dense granules disappear just before parturition; a time when relaxin levels drop. These results are interpreted as suggesting that the granules are a site of relaxin storage.