Molecular dissection of interspecific variation between Gossypium hirsutum and G-barbadense (cotton) by a backcross-self approach:: II.: Fiber fineness
Molecular dissection of interspecific variation between Gossypium hirsutum and G-barbadense (cotton) by a backcross-self approach:: II.: Fiber fineness
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DOI：
10.1007/s00122-005-2061-1
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发表时间：
2005-08-01
影响因子：
5.4
通讯作者：
Paterson, AH
中科院分区：
文献类型：
--
作者：
Draye, X;Chee, P;Paterson, AH
利用陆地棉（Gossypium hirsutum）与棉（G.以海岛棉（barbadense）为材料，分析了影响皮棉纤维细度的两个参数的遗传变异的分子基础，并比较了两种方法的测量精度。通过对来自24个独立的BC 3家系的3,662株BC 3F 2植株进行详细的限制性片段长度多态性（RFLP）分析，我们分别检测到了32个和9个纤维细度和马克隆值（MIC）的数量性状位点（QTL）。在这项研究中发现的数量较大的QTL比以前发现的其他研究的基础上F-2群体生长在有利的环境中反映了回交自我设计的能力，以解决较小的QTL效应。虽然这两种方法检测到的QTL数量差异很大，但9个MIC QTL中有7个与纤维细度相关。这支持了其他数据，表明纤维细度更准确地反映了棉纤维的基本物理特性，因此是选择的首选性状。“负超亲”现象，即大多数BC 3F 2家系表现出的平均表型比劣势亲本差，表明种间杂交形成的许多新基因组合是不适应的，可能是导致G.海岛棉在常规育种计划中的应用，以改善高地棉。
A backcross-self population from a cross between Gossypium hirsutum and G. barbadense was used to dissect the molecular basis of genetic variation governing two parameters reflecting lint fiber fineness and to compare the precision of these two measurements. By applying a detailed restriction fragment length polymorphism (RFLP) map to 3,662 BC3F2 plants from 24 independently derived BC3 families, we were able to detect 32 and nine quantitative trait loci (QTLs) for fiber fineness and micronaire (MIC), respectively. The discovery of larger numbers of QTLs in this study than previously found in other studies based on F-2 populations grown in favorable environments reflects the ability of the backcross-self design to resolve smaller QTL effects. Although the two measurements differed dramatically in the number of QTLs detected, seven of the nine MIC QTLs were also associated with fiber fineness. This supports other data in suggesting that fiber fineness more accurately reflects the underlying physical properties of cotton fibers and, consequently, is a preferable trait for selection. "Negative transgression," with the majority of BC3F2 families showing average phenotypes that were poorer than that of the inferior parent, suggests that many of the new gene combinations formed by interspecific hybridization are maladaptive and may contribute to the lack of progress in utilizing G. barbadense in conventional breeding programs to improve upland cotton.