Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria -: Possible modulation in vivo by thrombocytopenia
Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria -: Possible modulation in vivo by thrombocytopenia
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DOI：
10.1086/523761
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发表时间：
2008-01-01
影响因子：
6.4
通讯作者：
Grau, Georges Emile
中科院分区：
文献类型：
--
作者：
Wassmer, Samuel Crocodile;Taylor, Terrie;Grau, Georges Emile
疟原虫在人脑型疟疾（CM）的发病机制中可能起作用，并且它们已被证明在体外诱导恶性疟原虫寄生的红细胞（PRBCs）凝集。血小板减少症和血小板诱导的PRBC聚集与严重疟疾，特别是与CM相关。在本研究中，我们通过分离和共孵育CM患者的血浆和PRBCs体外，研究了CM患者中聚集现象的发生。马拉维CM患儿血小板计数均较低，血小板减少的程度与寄生虫血症的密度成正比。从这些患者获得的血浆样品随后诱导弱PRBC凝集。当重复测定时，将血浆血小板浓度调节至正常生理范围内，发生大量凝集。这项研究的结果表明，血小板减少症可能通过减少血小板介导的PRBC聚集，在CM期间为宿主提供保护机制。
Platelets may play a role in the pathogenesis of human cerebral malaria (CM), and they have been shown to induce clumping of Plasmodium falciparum-parasitized red blood cells (PRBCs) in vitro. Both thrombocytopenia and platelet-induced PRBC clumping are associated with severe malaria and, especially, with CM. In the present study, we investigated the occurrence of the clumping phenomenon in patients with CM by isolating and coincubating their plasma and PRBCs ex vivo. Malawian children with CM all had low platelet counts, with the degree of thrombocytopenia directly proportional to the density of parasitemia. Plasma samples obtained from these patients subsequently induced weak PRBC clumping. When the assays were repeated, with the plasma platelet concentrations adjusted to within the physiological range considered to be normal, massive clumping occurred. The results of this study suggest that thrombocytopenia may, through reduction of platelet-mediated clumping of PRBCs, provide a protective mechanism for the host during CM.