Enhanced immune response to foot-and-mouth disease vaccine by oral administration of ginseng stem-leaf saponins
Enhanced immune response to foot-and-mouth disease vaccine by oral administration of ginseng stem-leaf saponins
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口服人参茎叶皂苷增强口蹄疫疫苗免疫反应
DOI：
10.1080/08923973.2016.1184680
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发表时间：
2016-08-01
影响因子：
3.3
通讯作者：
Hu, Songhua
中科院分区：
文献类型：
--
作者：
Li, Renjun;Ma, Yanfen;Hu, Songhua
接种疫苗是控制口蹄疫的重要途径。研究了口服人参茎叶总皂苷(GSLS)对口蹄疫疫苗免疫应答和小鼠肠黏膜免疫功能的影响。在实验1中，小鼠灌胃给予GSLS或不给予GSLS作为对照。然后用口蹄疫疫苗对动物进行两次免疫。加强免疫后5周内每周采血一次，测定血清免疫球蛋白及其亚型。在实验2中，给小鼠灌胃GSLS或不给予GSLS作为对照。处死小鼠，取脾细胞进行淋巴细胞增殖试验，取肠道组织进行免疫组织化学和组织学检查。结果表明，口服GSLS可显著提高口蹄疫疫苗免疫小鼠的血清免疫球蛋白G和免疫球蛋白A(Ig A)+细胞的数量以及肠道上皮内淋巴细胞(IEL)和免疫球蛋白A(Ig A)+细胞的免疫应答。因此，GSLS可能是一种有效的口服佐剂，值得进一步研究以提高易感动物的疫苗接种率。
Vaccination is an important approach to the control of foot-and-mouth disease (FMD). This study evaluated the effect of oral administration of ginseng stem-leaf saponins (GSLS) on the immune response to FMD vaccine and the gut mucosal immunity in mice. In experiment 1, mice were orally administered GSLS or not treated as a control. The animals were then immunized twice with FMD vaccine. Blood was sampled weekly within five weeks after the boost immunization for measurement of serum IgG and the isotypes. In experiment 2, mice were orally administrated GSLS or not treated as a control. After that, splenocytes were prepared from sacrificed mice for lymphocyte proliferation assay and intestinal tissues were sampled for immunohistochemistry and histological examination. The results showed that oral administration of GSLS significantly enhanced serum IgG and the isotype responses to FMD vaccine as well as the number of intestinal intraepithelial lymphocytes (IELs) and immunoglobulin A (IgA)+cells. Therefore, GSLS may be a potent oral adjuvant and deserve further study to improve vaccination in susceptible animals.