Mass distribution and spatial organization of the linear bacterial motor of Spiroplasma citri R8A2.
Mass distribution and spatial organization of the linear bacterial motor of Spiroplasma citri R8A2.
复制标题
柑橘螺原体 R8A2 线性细菌运动的质量分布和空间组织。
DOI：
10.1128/jb.185.6.1987-1994.2003
复制
发表时间：
2003
影响因子：
3.2
通讯作者：
Leapman,RichardD
中科院分区：
文献类型：
--
作者：
Trachtenberg,Shlomo;Andrews,SBrian;Leapman,RichardD
在简单的螺旋形无壁细菌属螺原体中，趋化性和运动性受到线性收缩马达的影响，该马达排列为沿着沿着最短螺旋线附着于膜内侧的扁平细胞骨架带。通过扫描电镜和衍射分析，确定了柑橘螺原体R8 A2细胞骨架的层次结构和空间结构。结构单位似乎是一个纤维，约15 nm宽，由59 kDa蛋白质的二聚体组成;每个条带由7个纤维对组装而成。完整条带的功能单元是一对对齐的原纤维，沿着该对二聚体形成四聚环状重复。平均而言，分离和纯化的条带含有14个原纤维或7个排列良好的原纤维对，这是在完整细胞中观察到的相同结构。扫描透射电子显微镜质量分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳纯化的细胞骨架表明，59 kDa的蛋白质是唯一的组成部分的丝带。
In the simple, helical, wall-less bacterial genusSpiroplasma, chemotaxis and motility are effected by a linear, contractile motor arranged as a flat cytoskeletal ribbon attached to the inner side of the membrane along the shortest helical line. With scanning transmission electron microscopy and diffraction analysis, we determined the hierarchical and spatial organization of the cytoskeleton ofSpiroplasma citriR8A2. The structural unit appears to be a fibril, ∼5 nm wide, composed of dimers of a 59-kDa protein; each ribbon is assembled from seven fibril pairs. The functional unit of the intact ribbon is a pair of aligned fibrils, along which pairs of dimers form tetrameric ring-like repeats. On average, isolated and purified ribbons contain 14 fibrils or seven well-aligned fibril pairs, which are the same structures observed in the intact cell. Scanning transmission electron microscopy mass analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified cytoskeletons indicate that the 59-kDa protein is the only constituent of the ribbons.