Synthesis of D-Alanine Oligopeptides Catalyzed by D-Aminopeptidase in Non-Aqueous Media
Synthesis of D-Alanine Oligopeptides Catalyzed by D-Aminopeptidase in Non-Aqueous Media
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D-氨基肽酶在非水介质中催化 D-丙氨酸寡肽的合成
DOI：
10.3109/10242429008992063
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Kondo
中科院分区：
文献类型：
--
作者：
Y. Kato;Y. Asano;A. Nakazawa;K. Kondo
以人苍白杆菌（Achromobacter sp.）在非水介质中。当将250 mM底物与固定化D-氨肽酶（1.5 U/ml）和3当量三乙胺的水饱和甲苯溶液一起孵育3小时时，分别以56%和6%的产率获得D-丙氨酸二聚体和三聚体。该反应的kcat计算为19，500（min-1），比已知的氨基酸低聚物的酶促合成大数万倍。
Synthesis of D-alanine oligopeptides from D-alanine methylester hydrochloride has been demonstrated by use of immobilized D-aminopeptidase from Ochrobactrum anthropi (Achromobacter sp.) in non-aqueous media. D-Alanine dimer and trimer were obtained in 56% and 6% yield, respectively, when 250 mM of the substrate was incubated for 3 hours with urethane-prepolymer immobilized D-aminopeptidase (1.5 U/ml) and 3 equivalents of triethylamine in water-saturated toluene. The kcat of this reaction was calculated to be 19,500 (min−1), which is several ten thousand times greater than that of the known enzymatic syntheses of amino acid oligomers.