Genomic characterisation of two virulent Newcastle disease viruses isolated from crested ibis (Nipponia nippon) in China.
Genomic characterisation of two virulent Newcastle disease viruses isolated from crested ibis (Nipponia nippon) in China.
复制标题
DOI：
10.1016/j.gene.2014.09.059
复制
发表时间：
2014-12
期刊：
影响因子：
3.5
通讯作者：
Huafang Hao;Shengli Chen;Pengpeng Wu;Jie Wang;X. Duan;E. Du;Xinglong Wang;Zengqi Yang
中科院分区：
文献类型：
--
作者：
Huafang Hao;Shengli Chen;Pengpeng Wu;Jie Wang;X. Duan;E. Du;Xinglong Wang;Zengqi Yang
本文报道了新城疫病毒(NDV)两个强毒株陕西06(流行基因VIID)和陕西10(新亚型VII)的全基因组序列。两个分离株的基因组全长15,192个核苷酸，由6个基因组成，顺序为3‘-NP-P-M-F-HN-L-5’。这两个分离株的基因组与其他新城疫参考毒株高度相似。但在陕西06的HN蛋白和陕西10的F基因末端发现了一些独特的特征。陕系06和陕系10在F蛋白裂解位点具有相同的毒力基序112和−R-R-Q-K-R-F−117，这与已有的致病性测定结果一致。系统进化分析表明，两株新城疫病毒都属于II类新城疫病毒，其中陕西06株为VI型，陕西10株为VI型。两株毒株与流行于禽类和水禽中的新城疫流行毒株高度同源。这项研究首次提供了一种新的亚型新城疫病毒株和另一种亚型新城疫病毒的基因组信息，这将为后续的研究提供有用的信息。
This paper describes the complete genomic sequences of two virulent Newcastle disease virus (NDV) isolates, Shaanxi06 (prevalent genotype VIId) and Shaanxi10 (novel sub-genotype VIi), from sick crested ibises. The genomes of both isolates were 15,192 nt long and consisted of six genes in the order of 3′-NP-P-M-F-HN-L-5′. The genomes of the two isolates were highly similar to other reference NDV strains. However, some unique features were found in the HN protein of Shaanxi06 and the F gene end of Shaanxi10. Shaanxi06 and Shaanxi10 shared the same virulent motif112 −R-R-Q-K-R-F− 117at the F protein cleavage site, which coincided with previous pathogenicity test results. Phylogenetic analysis revealed that both isolates were clustered within class II NDV, with Shaanxi06 in genotype VII and Shaanxi10 in genotype VI. Both isolates shared high homology with the prevalent genotype NDV strains that circulate in fowls and waterfowls. This study is the first to provide genomic information about a novel sub-genotype VIi NDV strain and another genotype VIId virus, which will be useful for subsequent investigations.