Functional divergence of the bitter receptor TAS2R38 in Sulawesi macaques
Functional divergence of the bitter receptor TAS2R38 in Sulawesi macaques
复制标题
苏拉威西猕猴苦味受体 TAS2R38 的功能分化
DOI：
10.1002/ece3.5557
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发表时间：
2019
影响因子：
2.6
通讯作者：
Imai Hiroo
中科院分区：
文献类型：
--
作者：
Widayati Kanthi Arum;Yan Xiaochan;Suzuki‐Hashido Nami;Itoigawa Akihiro;Purba Laurentia Henrieta Permita Sari;Fahri Fahri;Terai Yohey;Suryobroto Bambang;Imai Hiroo
摘要苦味感知是由G蛋白偶联受体TAS 2 Rs介导的，通过诱导哺乳动物的先天性回避行为，在避免摄入毒素中起重要作用。研究得最好的TAS 2 R之一是TAS 2 R38，它介导合成苯硫脲（PTC）的苦味感知。以前的研究表明，TAS 2 R38的地理分离使苦味感知的独立分歧。尚未评估TAS 2 R38在异域物种中的功能差异。我们的特点TAS 2 R38的功能在四个异域物种的苏拉威西岛猕猴在苏拉威西。我们发现PTC味觉的变化在物种内和跨物种。在大多数情况下，TAS 2 R38是敏感的PTC，与物种之间的功能分歧。我们观察到不同的截短的TAS 2 R38，由于过早的终止密码子，它们在每个物种的Macaca nigra和M. nigrescens中对PTC没有反应。一些TAS 2 R38的氨基酸替换的完整变体显示出对东京烟草PTC的低敏感性。同样，这种具有PTC-低灵敏度的完整TAS 2 R38也在人体中发现。我们在所有四只苏拉威西猕猴中检测到一种共享的单倍型，这可能是苏拉威西猕猴的祖先单倍型。除了苏拉威西猕猴之间共享的单倍型外，其他TAS 2 R38单倍型具有物种特异性。这些结果表明，TAS 2 R38的变异可能受地理模式和当地适应性的影响。开放研究徽章这篇文章获得了开放数据徽章，因为它公开了复制报告结果所需的数字共享数据。数据可在https://doi.org/10.5061/dryad.908jf3r上查阅。
AbstractBitter perception is mediated by G protein‐coupled receptors TAS2Rs and plays an important role in avoiding the ingestion of toxins by inducing innate avoidance behavior in mammals. One of the best‐studied TAS2Rs is TAS2R38, which mediates the perception of the bitterness of synthetic phenylthiocarbamide (PTC). Previous studies of TAS2R38 have suggested that geographical separation enabled the independent divergence of bitter taste perception. The functional divergence of TAS2R38 in allopatric species has not been evaluated. We characterized the function of TAS2R38 in four allopatric species of Sulawesi macaques on Sulawesi Island. We found variation in PTC taste perception both within and across species. In most cases, TAS2R38 was sensitive to PTC, with functional divergence among species. We observed different truncated TAS2R38s that were not responsive to PTC in each species ofMacaca nigraandM. nigrescensdue to premature stop codons. Some variants of intact TAS2R38 with an amino acid substitution showed low sensitivity to PTC inM. tonkeana. Similarly, this intact TAS2R38 with PTC‐low sensitivity has also been found in humans. We detected a shared haplotype in all four Sulawesi macaques, which may be the ancestral haplotype of Sulawesi macaques. In addition to shared haplotypes among Sulawesi macaques, other TAS2R38 haplotypes were species‐specific. These results implied that the variation in TAS2R38 might be shaped by geographical patterns and local adaptation.OPEN RESEARCH BADGESThis article has earned an Open Data Badge for making publicly available the digitally‐shareable data necessary to reproduce the reported results. The data is available at https://doi.org/10.5061/dryad.908jf3r.