Identification of the Mtv-2 gene responsible for the early appearance of mammary tumors in the GR mouse by nucleic acid hybridization.
Identification of the Mtv-2 gene responsible for the early appearance of mammary tumors in the GR mouse by nucleic acid hybridization.
复制标题
通过核酸杂交鉴定导致 GR 小鼠乳腺肿瘤早期出现的 Mtv-2 基因。
DOI：
--
复制
发表时间：
1978
影响因子：
11.1
通讯作者：
R. van Nie
中科院分区：
文献类型：
--
作者：
R. Michalides;L. van Deemter;R. Nuss;R. van Nie
在GR小鼠品系中，Mtv-2基因控制第一次泌乳时乳汁中大量乳腺肿瘤病毒（MTV）抗原的表达。它还控制乳腺肿瘤的早期出现。我们已经研究了MTV前病毒序列的数量与此Mtv-2基因之间的核酸杂交MTV [（3）H]cDNA和DNA从GR，B10，和GR-Mtv-2（-）小鼠。B10和GR-Mtv-2（-）小鼠缺乏Mtv-2基因表达。分子杂交结果显示，GR小鼠DNA中含有12个拷贝的MTV前病毒序列，而B10和GR-Mtv-2（-）小鼠DNA中只有4个拷贝。因此，我们得出结论，Mtv-2基因在GR小鼠品系与8个额外的MTV前病毒序列。GR-Mtv-2（-）DNA中的四个Mtv前病毒序列可能代表GR小鼠中的另一个Mtv基因。在B10和GR-Mtv-2（-）小鼠的第一次泌乳时，在乳腺中检测到不同量的MTV RNA，尽管两者都含有四个拷贝的MTV前病毒序列。这表明这两种小鼠品系在这些MTV前病毒序列的表达调节或这些序列在鼠基因组中的位置方面存在差异。
In the mouse strain GR, the Mtv-2 gene controls the expression of large amounts of mammary tumor virus (MTV) antigens in the milk at first lactation. It also controls the early appearance of mammary tumors. We have investigated the number of MTV proviral sequences associated with this Mtv-2 gene by nucleic acid hybridization between MTV [(3)H]cDNA and DNA from GR, B10, and GR-Mtv-2(-) mice. B10 and GR-Mtv-2(-) mice lack Mtv-2 gene expression. The molecular hybridizations revealed that the DNA of GR mice contains 12 copies of MTV proviral sequences, whereas only 4 copies are present in the DNA of B10 and GR-Mtv-2(-) mice. We therefore conclude that the Mtv-2 gene in the GR mouse strain is associated with eight additional MTV proviral sequences. The four Mtv proviral sequences in the GR-Mtv-2(-) DNA might represent another Mtv gene in the GR mouse. Different amounts of MTV RNA are detected in mammary glands at first lactation of B10 and GR-Mtv-2(-) mice, even though both contain four copies of MTV proviral sequences. This indicates a difference between these two mouse strains either in the regulation of expression of these MTV proviral sequences or in the location of these sequences in the murine genome.