Molecular Toxicity of Metal Oxide Nanoparticles in Danio rerio
Molecular Toxicity of Metal Oxide Nanoparticles in Danio rerio
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DOI：
10.1021/acs.est.8b01464
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发表时间：
2018-07-17
影响因子：
11.4
通讯作者：
Wang, Xiangke
中科院分区：
文献类型：
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作者：
Hou, Jing;Liu, Haigiang;Wang, Xiangke
金属氧化物纳米颗粒会对人类和水生生物产生不利影响；然而，它们的毒性机制仍不清楚。我们使用微阵列分析和彗星测定研究了氧化铜、氧化锌和氧化镍纳米颗粒对斑马鱼的毒性作用和机制。氧化铜纳米粒子比其他金属氧化物纳米粒子更致命。对暴露于所有三种金属氧化物纳米颗粒后差异表达的基因进行基因本体分析表明，纳米颗粒主要通过改变核酸结合来影响细胞核中的核酸代谢。 KEGG分析将差异表达基因分类为遗传毒性相关途径“细胞周期”、“范可尼贫血”、“DNA复制”和“同源重组”。金属氧化物纳米颗粒的毒性可能与 DNA 合成和修复受损以及活性氧产生增加有关。
Metal oxide nanoparticles can exert adverse effects on humans and aquatic organisms; however, their toxic mechanisms are still unclear. We investigated the toxic effects and mechanisms of copper oxide, zinc oxide, and nickel oxide nanoparticles in Danio rerio using microarray analysis and the comet assay. Copper oxide nanoparticles were more lethal than the other metal oxide nanoparticles. Gene ontology analysis of genes that were differentially expressed following exposure to all three metal oxide nanoparticles showed that the nanoparticles mainly affected nucleic acid metabolism in the nucleus via alterations in nucleic acid binding. KEGG analysis classified the differentially expressed genes to the genotoxicity-related pathways "cell cycle", "Fanconi anemia", "DNA replication", and "homologous recombination". The toxicity of metal oxide nanoparticles may be related to impairments in DNA synthesis and repair, as well as to increased production of reactive oxygen species.