Integration host factor binds specifically to multiple sites in the ompB promoter of Escherichia coli and inhibits transcription.
Integration host factor binds specifically to multiple sites in the ompB promoter of Escherichia coli and inhibits transcription.
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整合宿主因子特异性结合大肠杆菌 ompB 启动子中的多个位点并抑制转录。
DOI：
10.1128/jb.173.18.5800-5807.1991
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发表时间：
1991
影响因子：
3.2
通讯作者：
Freundlich,M
中科院分区：
文献类型：
--
作者：
Tsui,P;Huang,L;Freundlich,M
大肠杆菌整合宿主因子（Escherichia coli integration host factor， IHF）是一种dna结合蛋白，在大肠杆菌及其部分噬菌体和质粒中参与基因调控、位点特异性重组等过程。在本研究中，我们发现IHF是大肠杆菌ompB操纵子的直接负效应。凝胶阻滞实验和dna酶I足迹研究表明，IHF与ompB启动子区域的三个位点结合。体外ompB启动子片段的转录被IHF特异性阻断。体内实验表明，IHF是生长细胞中ompB表达的负效应因子。对IHF结合位点突变的分析强烈表明，IHF在ompB启动子区域的结合是体内观察到的负面效应所必需的。
Escherichia coli integration host factor (IHF) is a DNA-binding protein that participates in gene regulation, site-specific recombination, and other processes in E. coli and some of its bacteriophages and plasmids. In the present study, we showed that IHF is a direct negative effector of the ompB operon of E. coli. Gel retardation experiments and DNase I footprinting studies revealed that IHF binds to three sites in the ompB promoter region. In vitro transcription from ompB promoter fragments was specifically blocked by IHF. In vivo experiments showed that IHF is a negative effector of ompB expression in growing cells. Analysis of IHF binding site mutations strongly suggested that IHF binding in the ompB promoter region is necessary for the negative effects seen in vivo.