Transposition of the Drosophila element mariner into the chicken germ line
Transposition of the Drosophila element mariner into the chicken germ line
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DOI：
10.1038/3497
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发表时间：
1998-11-01
影响因子：
46.9
通讯作者：
Sang, H
中科院分区：
文献类型：
--
作者：
Sherman, A;Dawson, A;Sang, H
果蝇转座因子mariner在鸡体内转座的能力是用一种携带有活性mariner因子的质粒注射到鸡受精卵中进行检测的。分析存活的胚胎和小鸡是否存在mariner。对存活至少12天的胚胎的分析表明，水手已经以高频率转座到鸡的基因组中，三只存活鸟类之一的水手的生殖系传播证实了转座。对第一代（G（1））小鸡的分析表明，它们每只都含有一到三个mariner拷贝。在G（1）鸟类中存在6种不同的转座事件，并且转座是由Mariner元件的转座酶基因的表达催化的，从G（1）到G（2）的传递以1：1的比例发生，因此Mariner具有开发为鸟类转基因载体的潜力。
The ability of the Drosophila transposable element mariner to transpose in the chicken was tested using a plasmid carrying an active mariner element injected into chick zygotes. Surviving embryos and chicks were analyzed for presence of mariner. Analysis of embryos that survived for at least 12 days of development indicated that mariner had transposed at high frequency into the chicken genome, Germline transmission of mariner from one of three surviving birds confirmed transposition. Analysis of the first-generation (G(1)) chicks showed that they each contained between one and three copies of mariner. Six different transposition events were represented in the G(1) birds, and the transposition was catalyzed by expression of the mariner element's transposase gene, Transmission from G(1) to G(2) occurred at a 1:1 ratio, Mariner therefore has potential for development as a vector for transgenesis in avian species.