A rapid screening technique for the detection of repeated DNA sequences in plant tissues
A rapid screening technique for the detection of repeated DNA sequences in plant tissues
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一种检测植物组织中重复 DNA 序列的快速筛选技术
DOI：
10.1007/bf00662455
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发表时间：
1985
影响因子：
5.4
通讯作者：
R. Flavell
中科院分区：
文献类型：
--
作者：
J. Hutchinson;A. Abbott;M. O. Dell;R. Flavell
从核染色体和线粒体染色体克隆的 DNA 序列已被用作杂交探针来区分不同的植物基因型。探针与组织的压扁片段杂交，例如组织。根提示。 “压点”法速度快，适合筛选大量单株植物。一种针对黑麦重复序列家族的探针能够在小麦植株中检测到黑麦染色体。核糖体 DNA 探针能够区分核糖体 RNA 基因数量多或少的植物。玉米线粒体 DNA 探针用于区分具有 N、T 或 S 细胞质的植物。
DNA sequences cloned from nuclear and mitochondrial chromosomes have been used as hybridisation probes to distinguish different plant genotypes. The probes are hybridised to squashed segments of tissue e.g. root tips. The ‘squash-dot’ method is rapid and suitable for screening large numbers of individual plants. One probe, specific for a rye repeated sequence family, enables rye chromosomes to be detected in wheat plants. A probe for ribosomal DNA enables plants with high or low numbers of ribosomal RNA genes to be distinguished. A maize mitochondrial DNA probe is used to distinguish plants with N, T or S cytoplasms.