Enteric fungal microbiota dysbiosis and ecological alterations in colorectal cancer
Enteric fungal microbiota dysbiosis and ecological alterations in colorectal cancer
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DOI：
10.1136/gutjnl-2018-317178
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发表时间：
2019-04-01
期刊：
影响因子：
24.5
通讯作者：
Yu, Jun
中科院分区：
文献类型：
--
作者：
Coker, Olabisi Oluwabukola;Nakatsu, Geicho;Yu, Jun
目的大肠癌与细菌组和病毒组的改变有关。然而，CRC中的肠道真菌微生物群在很大程度上仍未被探索。我们的目的是研究大肠癌中的肠道菌群。设计分析了来自香港的184例CRC患者、197例腺瘤患者和204例对照受试者的粪便鸟枪宏基因组序列（发现队列：73例CRC患者和92例对照受试者;验证队列：111例CRC患者、197例腺瘤患者和112例对照受试者）。CRC相关的真菌标志物和生态变化也在来自德国和法国的90例CRC患者、42例腺瘤患者和66例对照受试者的额外独立队列中进行了验证。结果主成分分析显示大肠癌和对照组（p <0.05）有不同的聚类，大肠癌和对照组（p <0.05）有显著性差异（p <0.05）。
Objectives Bacteriome and virome alterations are associated with colorectal cancer (CRC). Nevertheless, the gut fungal microbiota in CRC remains largely unexplored. We aimed to characterise enteric mycobiome in CRC.Design Faecal shotgun metagenomic sequences of 184 patients with CRC, 197 patients with adenoma and 204 control subjects from Hong Kong were analysed (discovery cohort: 73 patients with CRC and 92 control subjects; validation cohort: 111 patients with CRC, 197 patients with adenoma and 112 controls from Hong Kong). CRC-associated fungal markers and ecological changes were also validated in additional independent cohorts of 90 patients with CRC, 42 patients with adenoma and 66 control subjects of published repository sequences from Germany and France. Assignment of taxonomies was performed by exact k-mer alignment against an integrated microbial reference genome database.Results Principal component analysis revealed separate clusters for CRC and control (p