Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury
Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury
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DOI：
10.1016/j.bbrc.2007.05.029
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发表时间：
2007-07-13
影响因子：
3.1
通讯作者：
Cortright, D. N.
中科院分区：
文献类型：
--
作者：
Frederick, J.;Buck, M. E.;Cortright, D. N.
温敏TRP通道显示独特的热响应，表明介导温度的感觉传递的不同角色。然而，这些通道在背根神经节（DRG）中的相对表达是否在神经损伤中改变尚不清楚。我们开发了一种多重核糖核酸酶保护试验（RPA），以定量大鼠DRG中TRPV 1，TRPV 2，TRPV 3，TRPV 4，TRPA 1和TRPM 8 RNA水平。我们使用多重RPA来测量RTX治疗大鼠（300 μ g/kg）或单侧坐骨神经慢性压迫性损伤（CCI）大鼠DRG中的热敏TRP通道RNA水平。RTX处理的大鼠DRG中TRPV 1和TRPA 1 RNA显著降低，表明TRPA 1和TRPV 1在感觉伤害感受器中功能共定位。在CCI大鼠的DRG中，TRPA 1、TRPV 2和TRPM 8 RNA在周围神经损伤同侧显示轻微但显著的增加。我们的研究结果支持这一假设，增加TRP通道表达的感觉神经元可能有助于机械和冷超敏反应。(C)2007年爱思唯尔公司All rights reserved.
Thermosensitive TRP channels display unique thermal responses, suggesting distinct roles mediating sensory transmission of temperature. However, whether relative expression of these channels in dorsal root ganglia (DRG) is altered in nerve injury is unknown. We developed a multiplex ribonuclease protection assay (RPA) to quantify rat TRPV1, TRPV2, TRPV3, TRPV4, TRPA1, and TRPM8 RNA levels in DRG. We used the multiplex RPA to measure thermosensitive TRP channel RNA levels in DRG from RTX-treated rats (300 mu g/kg) or rats with unilateral sciatic nerve chronic constriction injury (CCI). TRPV1 and TRPA1 RNA were significantly decreased in DRG from RTX-treated rats, indicating functional colocalization of TRPA1 and TRPV1 in sensory nociceptors. In DRG from CCI rats, TRPA1, TRPV2, and TRPM8 RNA showed slight but significant increases ipsilateral to peripheral nerve injury. Our findings support the hypothesis that increased TRP channel expression in sensory neurons may contribute to mechanical and cold hypersensitivity. (C) 2007 Elsevier Inc. All rights reserved.