Protease amperometric sensor
Protease amperometric sensor
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DOI：
10.1021/ac060253w
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发表时间：
2006-09-15
影响因子：
7.4
通讯作者：
Marks, Robert S.
中科院分区：
文献类型：
--
作者：
Ionescu, Rodica E.;Cosnier, Serge;Marks, Robert S.
研制了一种检测胰蛋白酶的电流型生物传感器。后者是基于两层结构，即，聚合物-葡萄糖氧化酶内层和明胶外层。在葡萄糖的存在下，酶层产生H2O2，因此通过将电极恒电位在0.6 V产生由于H2O2电氧化引起的电流信号。生物传感器检测由明胶的蛋白水解消化引起的最大电流增加的变化，其覆盖铂电极，从而促进葡萄糖底物更快地接近用聚（吡咯-烷基铵）和葡萄糖氧化酶分子修饰的电极。我们的生物传感器检测到低胰蛋白酶浓度下降到42 pM的响应时间类似于10分钟，使其成为一个非常敏感的设备，在检测较低的胰蛋白酶水平与胰腺疾病的诊断等未来推定的应用。
An amperometric biosensor for the detection of trypsin was developed. The latter was based on a two-layer configuration, namely, a polymer-glucose oxidase inner layer and a gelatin outer layer. In the presence of glucose, the enzyme layer produces H2O2 and hence an amperometric signal due to H2O2 electrooxidation was generated by potentiostating the electrode at 0.6 V. The biosensor detects the change in the increase in the maximum current caused by the proteolytic digestion of gelatin, which covers the platinum electrodes, thereby facilitating a speedier access for the glucose substrate to the electrode modified with both poly(pyrrole-alkylammonium) and glucose oxidase molecules. Our biosensor detected low trypsin concentrations down to 42 pM with a response time of similar to 10 min, making it a very sensitive device in the detection of lower trypsin levels with such future putative applications as the diagnosis of pancreatic diseases.