THE EFFECT ON SYNAPTIC PHYSIOLOGY OF SYNAPTOTAGMIN MUTATIONS IN DROSOPHILA
THE EFFECT ON SYNAPTIC PHYSIOLOGY OF SYNAPTOTAGMIN MUTATIONS IN DROSOPHILA
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DOI：
10.1016/0896-6273(94)90342-5
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发表时间：
1994-04-01
期刊：
影响因子：
16.2
通讯作者：
SCHWARZ, TL
中科院分区：
文献类型：
--
作者：
DIANTONIO, A;SCHWARZ, TL
Synaptotagmin是一种突触囊泡蛋白，与神经递质释放有关。对黑腹果蝇中该蛋白的四个突变等位基因的分子表征，使我们能够研究synaptotagmin在突触生理学中的作用以及其功能的一些结构要求。突触蛋白水平的降低导致眼睛和幼虫神经肌肉连接处突触功能的实质性改变。神经递质释放减少导致诱发突触电位减小。然而，自发量子事件的频率，而不是大小，同时增加。这些异常似乎不是继发于突触树突的可检测形态学改变。自发事件频率的增加不足以显著耗尽囊泡供应，从而导致传播减少。这些数据是在synaptotagmin的功能包括囊泡对接作用的模型背景下讨论的。
Synaptotagmin is a synaptic vesicle protein implicated in neurotransmitter release. Molecular characterization of four mutant alleles of this protein in Drosophila melanogaster has permitted an investigation of synaptotagmin's role in synaptic physiology and of some of the structural requirements for its function. Reduced levels of synaptotagmin resulted in a substantial alteration in synaptic function in the eye and at larval neuromuscular junctions. Decreased neurotransmitter release caused smaller evoked synaptic potentials. However, the frequency, but not the size, of spontaneous quantal events was simultaneously increased. These abnormalities do not appear to be secondary to a detectable morphological change in the arborization of the synapse. The increased frequency of spontaneous events was insufficient to deplete significantly the vesicle supply and thereby account for reduced transmission. These data are discussed in the context of models in which synaptotagmin's function includes a role in vesicle docking.