Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells
Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells
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DOI：
10.3390/ijms141122721
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发表时间：
2013-11-01
影响因子：
5.6
通讯作者：
Kondo, Takashi
中科院分区：
文献类型：
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作者：
Tabuchi, Yoshiaki;Sugahara, Yuuki;Kondo, Takashi
虽然低强度脉冲超声(LIPUS)已被证明能促进骨折愈合，但其潜在机制仍未完全阐明。在这里，为了更好地了解LIPUS对细胞反应的分子机制，我们使用高密度寡核苷酸微阵列和计算基因表达分析工具，研究了LIPUS暴露于小鼠MC3T3-E1前成骨细胞的基因表达谱。LIPUS(1.5 MHz，30 mW/cm(2))单独作用20min，对细胞生长和碱性磷酸酶活力无明显影响，但可显著提高BGLAP的mRNA水平。基因芯片分析显示，处理后24小时，细胞内38个基因表达上调，37个基因表达下调1.5倍或以上。独创性通路分析表明，基因网络U(UP)中含有许多上调基因，这些基因主要与骨骼和肌肉系统发育和功能的生物学功能中的骨骼形态相关。此外，包含下调基因的基因网络D(Down)的生物学功能与基因表达、细胞周期和结缔组织的发育和功能有关。这些结果将有助于进一步阐明成骨细胞中LIPUS反应机制的分子基础。
Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm(2)) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells.