Development of a technique for measuring the oxidation rate of a 14C-labelled substrate from 14CO2 production without the need for collection of expired air.
Development of a technique for measuring the oxidation rate of a 14C-labelled substrate from 14CO2 production without the need for collection of expired air.
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开发了一种无需收集呼出空气即可测量 14CO2 生产中 14C 标记底物氧化速率的技术。
作者：
M. Clague;M. Keir;P. Wright
1. 提出了一种仅通过血液采样测定人体 14C 标记底物氧化速率的方法。 2.通过使用恒定速率输注[14C]碳酸氢钠模拟14C释放到患者碳酸氢盐池中来验证该方法，血浆总14CO2水平(d.p.m. ml-1 70 kg-1)与输注速率(microCi/h)良好相关(r = 0.878，P = 0.001)。 3. 讨论了使用该方法所涉及的技术，特别是考虑到所涉及的错误。
1. A method is proposed for the determination of the rate of oxidation of 14C-labelled substrates in man from blood sampling only. 2. The method was verified by simulating release of 14C into the bicarbonate pool of patients by using a constant-rate infusion of sodium [14C]bicarbonate, the plasma level of total 14CO2 (d.p.m. ml-1 70 kg-1) correlating well (r = 0.878, P = 0.001) with the infusion rate (microCi/h). 3. Techniques involved in employing this method are discussed, particularly in the light of errors involved.