Humoral immune response to the hypervariable region of hepatitis C virus differs between genotypes 1b and 2a.
Humoral immune response to the hypervariable region of hepatitis C virus differs between genotypes 1b and 2a.
复制标题
基因型 1b 和 2a 之间对丙型肝炎病毒高变区的体液免疫反应不同。
DOI：
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发表时间：
1997
影响因子：
6.4
通讯作者：
S. Kakumu
中科院分区：
文献类型：
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作者：
K. Yoshioka;T. Aiyama;A. Okumura;M. Takayanagi;K. Iwata;T. Ishikawa;Y. Nagai;S. Kakumu
抗丙型肝炎病毒高变区(HVR)抗体被认为具有中和活性。已知丙型肝炎病毒的基因会影响感染的临床病程。本文对21例慢性丙型肝炎患者进行了高变区抗体反应及其与病毒学和临床特征的关系研究。每个血清样本获得8~34个克隆(共375个克隆)，用免疫印迹法检测血清中的抗HVR抗体。6例2a型患者的抗HVR抗体阳性率和抗体活性均显著高于15例1b型患者。这一结果表明，对HVR的体液反应量影响感染这两种丙型肝炎病毒的临床结局。
Antibody to the hypervariable region (HVR) of HCV is thought to have neutralizing activity. The HCV genotype is known to affect the clinical course of infection. The antibody response to HVR and its relationship to the virologic and clinical characteristics were investigated in 21 patients with chronic hepatitis C. HVRs amplified by polymerase chain reaction from serum HCV were expressed as glutathione S-transferase fusion proteins. From 8 to 34 clones per serum sample were obtained (375 clones total), and the anti-HVR antibody in serum was assessed by Western blot. Both the incidence of fusion proteins positive for anti-HVR antibody and the activity of antibody were significantly higher in 6 patients with genotype 2a than in 15 patients with genotype 1b. This result suggests that the quantity of humoral response to HVR affects the clinical outcome of infection with these two HCV genotypes.