KINETIC-ANALYSIS OF DEOXYRIBONUCLEASE-I CLEAVAGES IN NUCLEOSOME CORE - EVIDENCE FOR A DNA SUPERHELIX
KINETIC-ANALYSIS OF DEOXYRIBONUCLEASE-I CLEAVAGES IN NUCLEOSOME CORE - EVIDENCE FOR A DNA SUPERHELIX
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DOI：
10.1016/0022-2836(78)90306-6
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发表时间：
1978-01-01
影响因子：
5.6
通讯作者：
LUTTER, LC
中科院分区：
文献类型：
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作者：
LUTTER, LC
使用DNase I消化标记在其DNA的5“或3”末端的核小体核心，确定DNase I敏感位点沿着140碱基对核小体核心（来自大鼠肝细胞核）DNA的位置。在每个站点的酶攻击的频率也确定从动力学分析的数据。DNA单链上间隔10 n个碱基（n是整数）的大多数位点都受到了攻击，但攻击的速度差别很大。相对攻击率的模式反映了DNA超螺旋的2圈上的位点的共同保护或暴露，该超螺旋每圈约有80个碱基对，并且可以与X射线晶体学研究相关联。此外，从链的一端到另一端的攻击频率的不对称分布可以用左手超螺旋来解释。
The location of the DNase I-sensitive sites along the DNA of the 140 base-pair nucleosome core [from rat liver nuclei] were determined using DNase I to digest nucleosome cores labeled at either the 5'' or 3'' ends of their DNA. The frequency of enzyme attack at each site was also determined from a kinetic analysis of the data. Most of the sites spaced 10n bases apart (where n is an integer) on a single strand of the DNA were attacked, but at widely differing rates. The pattern of relative rates of attack reflects the common protection or exposure of sites on the 2 turns of a DNA super helix which has about 80 base-pairs per turn and can be correlated with X-ray crystallographic studies. Furthermore, the asymmetric distribution of frequencies of attack from one end of a strand to the other can be interpreted in terms of a left-handed super helix.