Ganglioside analysis by thin-layer chromatography matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry
Ganglioside analysis by thin-layer chromatography matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry
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DOI：
10.1016/j.jasms.2005.05.003
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发表时间：
2005-09-01
影响因子：
3.2
通讯作者：
Costello, CE
中科院分区：
文献类型：
--
作者：
Ivleva, VB;Sapp, LM;Costello, CE
基质辅助激光解吸/电离（MALDI）质谱仪中产生的离子的亚稳态分解使具有不稳定键的生物样品的分析复杂化。最近，几个学术实验室和商业仪器制造商已经设计出在MALDI事件期间将冷却气体引入离子源的仪器，并且已经表明所产生的振动冷却稳定了这些不稳定的键。在这项研究中，我们比较了稳定和检测解吸神经节苷脂的商业orthogonaltime-of-flight（oTOF）仪器与我们以前报道的结果，已经获得了一个自制的傅里叶变换质谱仪。从检测步骤的解吸附/电离的解耦导致直接从TLC板解吸附薄层色谱（TLC）分离的神经节苷脂的机会，而不损害神经节苷脂分析的质谱准确度和分辨率，因此耦合TLC和oTOF质谱。去簇电位的应用允许控制基质簇和基质加合物的形成，并且因此增强了神经节苷脂的检测。(c)2005年美国质谱学会。
Metastable decomposition of ions generated in matrix-assisted laser desorption/ionization (MALDI) mass spectrometers complicates analysis of biological samples that have labile bonds. Recently, several academic laboratories and manufacturers of commercial instruments have designed instruments that introduce a cooling gas into the ion source during the MALDI event and have shown that the resulting vibrational cooling stabilizes these labile bonds. In this study, we compared stabilization and detection of desorbed gangliosides on a commercial orthogonaltime-of-flight (oTOF) instrument with results we reported previously that had been obtained on a home-built Fourier transform mass spectrometer. Decoupling of the desorption/ionization from the detection steps resulted in an opportunity for desorbing thin-layer chromatography (TLC)-separated gangliosides directly from a TLC plate without compromising mass spectral accuracy and resolution of the ganglioside analysis, thus coupling TLC and oTOF mass spectrometry. The application of a declustering potential allowed control of the matrix cluster and matrix adduct formation, and, thus, enhanced the detection of the gangliosides. (c) 2005 American Society for Mass Spectrometry.