Identification and analysis of the stigma and embryo sac-preferential/specific genes in rice pistils.
Identification and analysis of the stigma and embryo sac-preferential/specific genes in rice pistils.
复制标题
水稻雌蕊柱头和胚囊优先/特异性基因的鉴定与分析
DOI：
10.1186/s12870-017-1004-8
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发表时间：
2017-03-07
影响因子：
5.3
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Yu L;Ma T;Zhang Y;Hu Y;Yu K;Chen Y;Ma H;Zhao J
水稻雌蕊是雌性生殖器官，由两个柱头和一个子房组成。柱头能够接受花粉粒并引导花粉管生长。子房内有胚囊，胚囊与雄配子受精产生种子。然而，很少有人知道在这些过程中的基因功能和调控网络在rice.ResultsHere，使用RNA-Seq技术，我们确定了3531柱头优先基因和703柱头特异基因在水稻雌蕊，我们验证了13个柱头特异基因通过qRT-PCR和原位杂交。GO分析表明，柱头中的运输、定位、膜、通讯和授粉相关基因均显著富集。此外，为了确定胚囊优先/雌蕊内的特定基因，我们比较了一个野生型的子房与一个muglidst（有缺陷的柱头）子房，发现385个基因下调indst。在这些基因中，122个表现出子房特异性表达模式，被认为是胚囊优先/雌蕊内的特异性基因。其中大部分基因在雌蕊中优先表达，14个基因在雌蕊中特异表达。此外，一些在有性生殖过程中起重要作用的水稻胚囊特异性基因的同源基因在水稻中表达下调。此外，我们确定了102个新的蛋白质编码基因，其中6个表现出不同的柱头和子房在水稻中确定使用RT-PCR.ConclusionsAccording这些水稻子房比较，许多基因优先或特异性表达的柱头，这表明他们参与柱头发育或授粉。GO分析表明，水稻干燥柱头可能主要通过细胞膜来发挥其功能，这与其他物种的湿润柱头不同。此外，在雌蕊中还发现了许多胚囊优先/特异基因，这些基因可能在水稻雌配子体中表达，暗示这些基因可能参与雌配子体的特化和受精过程。为研究雌配子体发育和受精过程中的基因功能和调控网络提供了基因信息。此外，这些新基因对于补充和完善水稻中现有的转录组具有重要价值，为水稻新基因的检测提供了一种有效的方法。
BackgroundIn rice, the pistil is the female reproductive organ, and it consists of two stigmas and an ovary. The stigma is capable of receiving pollen grains and guiding pollen tube growth. The ovary holds the embryo sac, which is fertilized with male gametes to produce seed. However, little is known about the gene function and regulatory networks during these processes in rice.ResultsHere, using the RNA-Seq technique, we identified 3531 stigma-preferential genes and 703 stigma-specific genes within the rice pistils, and we verified 13 stigma-specific genes via qRT-PCR andin situhybridization. The GO analysis showed that the transport-, localization-, membrane-, communication-, and pollination-related genes were significantly enriched in the stigma. Additionally, to identify the embryo sac-preferential/specific genes within the pistils, we compared a wild-type ovary with a mutantdst(defective stigma) ovary and found that 385 genes were down-regulated indst. Among these genes, 122 exhibited an ovary-specific expression pattern and are thought to be embryo sac-preferential/specific genes within the pistils. Most of them were preferentially expressed, while 14 of them were specifically expressed in the pistil. Moreover, the rice homologs of someArabidopsisembryo sac-specific genes, which played essential roles during sexual reproduction, were down-regulated indst. Additionally, we identified 102 novel protein-coding genes, and 6 of them exhibited differences between the stigma and ovary in rice as determined using RT-PCR.ConclusionsAccording to these rice ovary comparisons, numerous genes were preferentially or specifically expressed in the stigma, suggesting that they were involved in stigma development or pollination. The GO analysis indicated that a dry rice stigma might primarily perform its function through the cell membrane, which was different from the wet stigma of other species. Moreover, many embryo sac-preferential/specific genes within the pistils were identified and may be expressed in female rice gametophytes, implying that these genes might participate in the process of female gametophyte specialization and fertilization. Therefore, we provide the gene information for investigating the gene function and regulatory networks during female gametophyte development and fertilization. In addition, these novel genes are valuable for the supplementation and perfection of the existing transcriptome in rice, which provides an effective method of detecting novel rice genes.
烟草受精卵两个子细胞的比较转录谱分析揭示了顶端细胞和基底细胞的转录组差异
影响因子：
5.3
作者：
Hu TX;Yu M;Zhao J
通讯作者：
Zhao J