Patterns and persistence of SARS-CoV-2 IgG antibodies in Chicago to monitor COVID-19 exposure
Patterns and persistence of SARS-CoV-2 IgG antibodies in Chicago to monitor COVID-19 exposure
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DOI：
10.1172/jci.insight.146148
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发表时间：
2021-05-10
期刊：
影响因子：
8
通讯作者：
McNally, Elizabeth M.
中科院分区：
文献类型：
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作者：
Demonbreun, Alexis R.;McDade, Thomas W.;McNally, Elizabeth M.
背景对SARS-CoV-2血清阳性率的估计差异很大，可能影响疫苗接种反应。我们确定了2020年6月至2020年12月期间美国大都市芝加哥的IgG水平。通过电子广告招募参与者（n = 7935），并通过邮件或最小接触亲自方法接收用于自采样干血斑试验的材料。IgG抗SARS-CoV-2的受体结合域的测定使用一个建立的高灵敏度和高特异性的检测。总体血清阳性率为17.9%，不同接触方法之间无显著差异。只有2.5%的参与者报告根据病毒检测诊断为COVID-19，这与通过血清学测量的SARS-CoV-2暴露量是通过病毒检测检测的7倍相一致。在这项社区调查中，血清反应阳性参与者中观察到的IgG水平范围与记录PCR检测阳性的COVID-19病例相关的IgG水平范围重叠。从参加重复检测的一个亚组中，半数血清学阳性个体保留可检测的抗体3至4个月。用高度特异性和灵敏度的检测方法进行的定量IgG测量表明，SARS-CoV-2的暴露范围比病毒检测所观察到的更广泛。这些无症状暴露产生的IgG浓度范围与记录的非住院COVID-19后产生的IgG水平相似，后者远低于住院COVID-19病例产生的IgG水平。IgG反应的不同范围，加上抗体的衰减率，可能会影响对随后的病毒暴露和疫苗的反应。
BACKGROUND. Estimates of seroprevalence to SARS-CoV-2 vary widely and may influence vaccination response. We ascertained IgG levels across a single US metropolitan site, Chicago, from June 2020 through December 2020.METHODS. Participants (n = 7935) were recruited through electronic advertising and received materials for a self-sampled dried-blood spot assay through the mail or a minimal contact in-person method. IgG against the receptor-binding domain of SARS-CoV-2 was measured using an established highly sensitive and highly specific assay.RESULTS. Overall seroprevalence was 17.9%, with no significant difference between method of contact. Only 2.5% of participants reported having had a diagnosis of COVID-19 based on virus detection, consistent with a 7-fold greater exposure to SARS-CoV-2 measured by serology than that detected by viral testing. The range of IgG level observed in seropositive participants from this community survey overlapped with the range of IgG levels associated with COVID-19 cases having a documented positive PCR test. From a subset of those who participated in repeat testing, half of seropositive individuals retained detectable antibodies for 3 to 4 months.CONCLUSION. Quantitative IgG measurements with a highly specific and sensitive assay indicated more widespread exposure to SARS-CoV-2 than observed by viral testing. The range of IgG concentrations produced from these asymptomatic exposures was similar to IgG levels occurring after documented nonhospitalized COVID-19, which were considerably lower than those produced from hospitalized COVID-19 cases. The differing ranges of IgG response, coupled with the rate of decay of antibodies, may influence response to subsequent viral exposure and vaccine.